论文部分内容阅读
目的：研究辅助性T细胞17（T helper cell 17， Th17）和调节性T细胞（regulatory T cell ， Treg ）及细胞因子在慢性阻塞性肺疾病（COPD）患者中的变化。方法抽取入选的COPD患者120例〔急性加重期（AECOPD）及稳定期COPD各60例〕和健康对照者240例外周血，以流式细胞术分析Th17和Treg淋巴细胞，同时用ELISA法检测其白细胞介素-17（ IL-17）表达水平，并对COPD患者行肺功能检查。结果①AECOPD 组和稳定期COPD组患者外周血Th17、IL-17及Treg/Th17水平明显高于正常对照组；②COPD患者Th17、IL-17与第1 s用力呼气量（ FEV1）呈正相关；③COPD患者Treg/Th17与肺功能无相关性。结论 COPD患者外周血存在Treg/Th17及IL-17变化。“,”Objective To investigate the changes of T helper ( Th) 17 cells, regulatory T cells ( Treg) and IL-17 in patients with chronic obstructive pulmonary disease ( COPD) .Methods Peripheral blood was collected from 120 pa-tients with COPD ( acute exacerbation of COPD and stable COPD , 60 cases for each ) and 240 healthy subjects .Then, the amounts of Th17 and Treg lymphocytes were analyzed by flow cytometry , while the level of IL-17 was detected by ELISA.Each COPD patient received pulmonary function examination .Results The levels of Th17, IL-17 and Treg/Th17 in patients with AECOPD or stable COPD were significantly higher than those in healthy individuals .The levels of Th17 and IL-17 were positively correlated with FEV 1 for COPD patients .No relationship was found between the ratio of Treg/Th17 and the pulmonary function of COPD patients .Conclusion Changes in the ratio of Treg/Th17 and the level of IL-17 exist in the peripheral blood of COPD patients .