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Kohler和Mistein(1975)首先使用了杂交瘤技术。它为研究细胞表面抗原提供了一种极好的工具。通过产生抗体细胞的克隆化,选择对单一抗原决定簇有特异性的单克隆抗体,可避免运用常规抗血清时反复吸收。在作者企图用异种抗血清的方法来解释巨噬细胞的异质性时,反复吸收却成为一种障碍。因此种方法产生的对单一抗原决定簇有特异性的抗血清效价太低,对进一步研究是无用的。因此,作者使用杂交瘤技术去研究大鼠巨噬细胞的抗原异质性。
Kohler and Mistein (1975) first used the hybridoma technique. It provides an excellent tool for studying cell surface antigens. By producing clones of antibody cells, the selection of monoclonal antibodies specific for a single antigenic determinant can avoid repeated uptake with conventional antisera. Repeated absorption is an obstacle when the author attempts to explain the heterogeneity of macrophages using a heterologous antiserum. Therefore, the titer of the antisera specific to a single antigenic determinant generated by the method is too low, which is useless for further research. Therefore, the authors used hybridoma technology to study the antigenic heterogeneity of rat macrophages.