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取高粱幼苗切去根部,削去外部叶鞘,露出生长锥,放在乳酚油锥蓝染剂(或棉蓝里)煮染20分钟左右,至材料软化为止,取出夹在两片载玻片里压开,在低倍显微镜下观察,病株生长锥和心叶细胞组织染成浅蓝色,丝黑穗菌菌丝染成深蓝色,呈一段段瘤节状分枝,易于识别。据两年田间对比试验结果,本法预测田间发病率比较准确、简易、快速,适用于室内药剂筛选和田间预测药剂效果。田间高粱出苗后,分期取病株以徒手切片染色观察,侵染菌丝先集中于生长锥基部,后移向生长点里,花芽分化后移进花穗里。成株的高粱病株的每节组织里,均有丝黑穗菌丝存在。丝黑穗菌从高粱幼芽芽鞘侵入,侵入时期从种子发茅到芽长1.5厘米。在高粱幼苗生长锥里的散黑穗菌和坚黑穗菌的菌丝与丝黑穗菌菌丝,在形态上不易区别。
Take sorghum seedlings cut to the root, cut the outer sheath, revealing the growth cone, on the milk phenol cones blue dye (or cotton blue) boiled for 20 minutes until the material softened, remove the clip in the two slides Press open, observed under low magnification microscope, the growth cone and leaf tissue of the diseased plants stained light blue, black smith mycelium stained dark blue, was a section of tumor nodular branches, easy to identify. According to the results of comparative experiments in two years in the field, this article predicts that the incidence rate in the field is accurate, simple and fast, which is suitable for indoor pharmacy screening and field forecast medicament effect. After field emergence of sorghum, the diseased plants were stained with bare hands for staging. The infected hyphae first focused on the base of growth cone and then moved to the growing point. After the flower buds were differentiated, they moved into the spikes. Sorghum strain of adult plants in each section, there are filamentous mycelium existence. Smut from sorghum sprouts sheath invasion, invasion from the seed to bud length of 1.5 cm. In the sorghum seedling growth cone black smut and black mycelium mycelium and silk mycelium mycelium is not easy to distinguish in morphology.