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目的 :研究和探讨突变型 p5 3、C- myc和血管内皮生长因子 (VEGF)基因蛋白在胶质瘤中的表达和在胶质瘤发生过程中的作用及临床病理意义。 方法:6 5例胶质瘤患者分为低级别组 ( ～ 级 ) 2 8例 ,高级别组 ( ～ 级 ) 37例 ,采用免疫组织化学法检测两组胶质瘤 p5 3、C- myc、VEGF 3种基因蛋白的表达 ,并进行统计学分析。结果 :突变型 p5 3、C- m yc和 VEGF在胶质瘤中的阳性表达率分别为 6 3% (39/6 2 )、6 0 % (35 /5 8)、72 % (4 4 /6 1)。两组VEGF、C- m yc的阳性表达率差异有统计学意义 (P <0 .0 1) ,突变型 p5 3的表达在两组间差异无统计学意义 (P >0 .0 5 ) ,VEGF的产生与 p5 3突变和 C- myc过度表达之间无相关性 (P >0 .0 5 )。结论:p5 3突变和 C- m yc过度表达在胶质瘤发生过程中起重要的作用 ,肿瘤组织产生和分泌 VEGF在胶质瘤快速生长、恶性增殖过程中起关键作用 ,通过阻断 VEGF或其受体抑制成血管过程 ,有望对临床治疗胶质瘤提供一种新途径。
Objective: To investigate and explore the expression of mutant p5 3, C-myc and vascular endothelial growth factor (VEGF) gene in glioma and its role in the pathogenesis of glioma and its clinicopathological significance. Methods: Sixty-five patients with glioma were divided into two groups: 28 cases in low grade group (~ grade) and 37 cases in high grade group (~ grade). Immunohistochemistry was used to detect the expression of p53, C- VEGF 3 kinds of gene protein expression, and statistical analysis. RESULTS: The positive rates of p5 3, C-myc and VEGF in gliomas were 63% (39/62), 60% (35/58), 72% (4 4 / 6 1). The positive rates of VEGF and C-mc in the two groups were significantly different (P <0.01). There was no significant difference between the two groups in the expression of mutant p5 (P> 0.05) There was no correlation between VEGF production and p5 3 mutation and C-myc overexpression (P> 0.05). CONCLUSION: The overexpression of p5-3 and C-y-yc play an important role in the pathogenesis of glioma. The production and secretion of VEGF in tumor tissue play a key role in the rapid growth and malignant proliferation of glioma. By blocking VEGF or Inhibition of its receptor into vascular processes, is expected to provide a new way for clinical treatment of glioma.