论文部分内容阅读
目的观察核素标记叶酸靶向白蛋白纳米微球对人卵巢癌细胞生长的影响。方法将体外培养的SKOV3人卵巢癌细胞分为八组:阴性对照组(只加RPMI-1640培养液),单纯化疗组(叶酸偶联载药白蛋白磁性纳米微球,不加磁场),单纯放疗组[188铼(188 Re)标记的叶酸偶联白蛋白磁性纳米微球,不加磁场],单纯热疗组(叶酸偶联白蛋白磁性纳米微球,加磁场),化疗联合放疗组(188 Re标记的叶酸偶联载药白蛋白磁性纳米微球,不加磁场),化疗联合热疗组(叶酸偶联载药白蛋白磁性纳米微球,加磁场),放疗联合热疗组(188 Re标记的叶酸偶联白蛋白磁性纳米微球,加磁场)和热疗、化疗、放疗联合治疗组(联合治疗组,188 Re标记的叶酸偶联载药白蛋白磁性纳米微球,加磁场)。48h后MTT方法测定各组细胞增殖率,流式细胞术测定细胞凋亡率。结果阴性对照组对卵巢癌细胞增殖的抑制作用弱于其他各组,联合治疗组强于其他各组(P<0.05)。单纯热疗组、化疗联合放疗组、化疗联合热疗组、放疗联合热疗组和联合治疗组中细胞周期G1期前出现明显的亚二倍体凋亡峰;阴性对照组、单纯热疗组、化疗联合放疗组、化疗联合热疗组、放疗联合热疗组和联合治疗组的细胞凋亡率分别为0.08%、7.56%、17.14%、21.64%、33.94%和57.16%。结论磁感应热疗、化疗、核素靶向放疗的联合作用能有效抑制人卵巢癌细胞的生长。
Objective To observe the effects of nuclease-labeled albumin-loaded albumin nanoparticles on the growth of human ovarian cancer cells. Methods SKOV3 human ovarian cancer cells cultured in vitro were divided into eight groups: negative control group (RPMI-1640 medium only), chemotherapy alone group (folic acid coupled drug-loaded albumin magnetic nanospheres without magnetic field), simple Radiotherapy group [188 Re labeled folate conjugated albumin magnetic nanosphere without magnetic field], hyperthermia group (folic acid conjugated albumin magnetic nanospheres plus magnetic field), chemotherapy combined with radiotherapy group 188 Re-labeled folate-conjugated drug-loaded albumin magnetic nanoparticles without magnetic field), chemotherapy combined with hyperthermia group (folic acid-conjugated drug-loaded albumin magnetic nanospheres plus magnetic field), radiotherapy combined with hyperthermia group Re-labeled folic acid conjugated albumin magnetic nanospheres with magnetic field) and hyperthermia, chemotherapy and radiotherapy (combination therapy group, 188 Re labeled folic acid-conjugated albumin magnetic nanospheres with magnetic field) . After 48h MTT method was used to determine the proliferation rate of each group, and the apoptosis rate was determined by flow cytometry. Results The negative control group showed less inhibitory effect on the proliferation of ovarian cancer cells than other groups. The combined treatment group was stronger than the other groups (P <0.05). The hyperthermia group, chemotherapy combined with radiotherapy group, chemotherapy combined hyperthermia group, radiotherapy combined with hyperthermia group and combination therapy group had obvious sub-diploid apoptotic peak before G1 phase. In the negative control group, hyperthermia group The rates of apoptosis of chemotherapy combined with radiotherapy, chemotherapy plus hyperthermia, radiotherapy plus hyperthermia and combination therapy were 0.08%, 7.56%, 17.14%, 21.64%, 33.94% and 57.16%, respectively. Conclusion The combined effect of magnetic induction hyperthermia, chemotherapy and radionuclide targeted radiotherapy can effectively inhibit the growth of human ovarian cancer cells.