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目的了解供澳门水产品中致病性弧菌的污染状况和三间分布。方法 2012年8月—2013年7月,采用随机抽样的方法收集珠海、中山两地的供澳水产品样品1 510份,利用基于HAND系统多重PCR方法初筛后采用常规分离培养和生化鉴定验证,分析其污染状况,季节性、地域性以及水产品种类的分布。结果供澳水产品样品中弧菌阳性率为59.5%(898/1 510),复合感染率为37.3%(563/1 510)。一年中的5~11月份致病性弧菌总阳性率较高,1月和12月阳性率较低,差异有统计学意义(P<0.05)。珠海地区致病性弧菌总阳性率(69.5%)高于中山(51.9%),差异有统计学意义(P<0.05)。贝类的致病性弧菌总阳性率(70.6%)高于鱼类(54.4%),差异有统计学意义(P<0.05)。结论供澳门水产品中致病性弧菌污染现象严重,需加大致病性弧菌的监测力度,以防致病性弧菌的感染和流行。
Objective To understand the status and distribution of pathogenic Vibrio species in aquatic products in Macao. Methods From August 2012 to July 2013, a total of 1 510 samples of aquatic products were collected from Zhuhai and Zhongshan using random sampling method. After screening by multiplex PCR based on HAND system, they were verified by routine isolation, culture and biochemical identification , Analyze its pollution status, seasonal, regional and aquatic species distribution. Results The positive rate of vibrios in samples of Australian aquatic products was 59.5% (898/1 510) and the composite infection rate was 37.3% (563/1 510). The total positive rate of pathogenic Vibrio was higher in May to November of the year, the positive rate was lower in January and December, the difference was statistically significant (P <0.05). The total positive rate of pathogenic Vibrio in Zhuhai (69.5%) was higher than that in Zhongshan (51.9%), with a significant difference (P <0.05). The positive rate of pathogenic Vibrio in shellfish was higher than that of fish (70.6%) (54.4%), the difference was statistically significant (P <0.05). Conclusion The pathogenic Vibrio parahaemolyticus in Macao aquatic products is seriously polluted. It is necessary to increase the monitoring of pathogenic Vibrio to prevent the infection and epidemic of pathogenic Vibrio.