论文部分内容阅读
目的 研究不同剂量左旋多巴对帕金森病大鼠及黑质形态学作用 ,探讨左旋多巴合理的方法。方法 通过 6- OHDA脑立体定向注射术建立大鼠帕金森病模型 ,采用行为学、TUNEL、原位杂交的方法观察左旋多巴不同剂量 (1 0 mg/ kg/ d、50 mg/ kg/ d、1 0 0 mg/ kg/ d)、不同的作用时间 (× 1 d、× 3 d、× 5 d、× 7d)对帕金森病大鼠黑质细胞的毒性作用 ,并观察治疗后 7d各项指标的变化。结果 PD大鼠旋转行为、黑质细胞凋亡数及 bcl- 2 m RNA随着左旋多巴治疗的时间、剂量增加而增加。除小剂量组外 ,中、大剂量组各项指标均高于对照组 (P<0 .0 5) ,停止治疗后 7d,左旋多巴治疗各组指标均得到改善。结论 左旋多巴能加速 PD大鼠黑质细胞凋亡 ,这种作用受到 bcl- 2 m RNA调控 ,小剂量、间隔使用左旋多巴能有效减少黑质细胞凋亡。
Objective To study the morphological effects of different doses of levodopa on Parkinson’s disease and substantia nigra in rats, and to explore the reasonable method of levodopa. Methods Parkinson ’s disease model was established by injecting 6 - OHDA stereotaxically in rats. The effects of levodopa at different dosages (10 mg / kg / d and 50 mg / kg / d) were observed by behavior, TUNEL and in situ hybridization , 100 mg / kg / d) for different duration of action (× 1 d, × 3 d, × 5 d, × 7 d) on the substantia nigra of Parkinson’s disease rats. Changes in the indicators. Results The rotational behavior, number of apoptotic cells and bcl-2 mRNA in PD rats were increased with the increase of levodopa treatment time and dose. Except for the low-dose group, the indexes of middle-dose and high-dose groups were higher than those of the control group (P <0.05). After 7 days of treatment, the indexes of levodopa treatment were improved. Conclusion Levodopa can accelerate the apoptosis of PD in rat substantia nigra. This effect is regulated by bcl-2 m RNA. L-DOPA can effectively reduce the apoptosis of substantia nigra in small dose and interval.