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目的 :观察一氧化氮及一氧化氮合酶抑制剂在大鼠局灶性脑缺血时的作用。方法 :用直接电凝大鼠大脑中动脉制成脑缺血模型 ,选择脑缺血 30 min、6 0 min、1 2 0 min、1 80 min为研究时点 ,观察各时点用选择性、非选择性一氧化氮合酶抑制剂 ( AG、L-NAME)亚硝酸盐含量测定、缺血脑组织坏死体积测定及损伤海马 CA1 区电镜观察。结果 :缺血组、用药组从 30分钟到 6 0分钟亚硝酸盐含量呈下降趋势 ,而从 6 0分钟至 1 80分钟又至上升趋势 ,病理及电镜显示 30分钟时用药组损伤重于缺血组 ,而 6 0分钟至 1 80分钟相反。结论 :NO在局灶缺血性脑损伤中呈现双重作用 ,一氧化氮合酶抑制剂对脑缺血后期治疗有效。
Objective: To observe the effect of nitric oxide and nitric oxide synthase inhibitors on focal cerebral ischemia in rats. Methods: The model of cerebral ischemia was established by direct coagulation of middle cerebral artery in rats. Cerebral ischemia was selected at 30 min, 60 min, 120 min and 180 min as study time points, Nitrite content of non-selective nitric oxide synthase inhibitor (AG, L-NAME), volumetric determination of ischemic brain tissue necrosis and electron microscopic observation of injured hippocampal CA1 area. Results: In the ischemic group, the content of nitrite decreased from 30 minutes to 60 minutes, and then increased from 60 minutes to 180 minutes. The pathology and electron microscopy showed that the damage of the medicine group was heavier than that of the control group at 30 minutes The blood group, while 60 minutes to 180 minutes instead. CONCLUSION: NO plays a dual role in focal ischemic brain injury. Nitric oxide synthase inhibitor is effective in the treatment of late stage of cerebral ischemia.