论文部分内容阅读
目的：检测ＨＢＶ携带者产后不同时期母乳ＨＢＶ－ＤＮＡ携带情况。方法：用２ｍｌ无菌离心管取最早初乳１．５ｍｌ用聚合酶链反应（ＰＣＲ）检测ＨＢＶ－ＤＮＡ。第１次初乳ＨＢＶ－ＤＮＡ阳性者于２４ｈ后的不同时间用同样方法第２次取母乳，用ＰＣＲ２次扩增技术检测乳汁中的ＨＢＶ－ＤＮＡ。结果：第１次检测２１９例ＨＢＶ携带者的初乳，１８例ＨＢＶ－ＤＮＡ阳性。这１８例最少间隔２４ｈ再取母乳检测，只有１例检测到ＨＢＶ－ＤＮＡ。结论：携带ＨＢＶ产妇下乳２４ｈ后的母乳ＨＢＶ－ＤＮＡ浓度明显低于最早初乳。因此ＨＢＶ携带者最好排掉下乳２４ｈ的初乳再行哺乳，可提高母乳喂养的安全性。
Objective: To detect the carrier of HBV-DNA in breast milk of HBV carriers at different stages of postnatal period. Methods: 1.5ml of the earliest colostrum was taken by 2ml sterile centrifuge tube and HBV-DNA was detected by polymerase chain reaction (PCR). The first colostrum HBV-DNA positive at different times after 24 hours by the same method to take the second breast milk, PCR2 amplification technology to detect milk in the HBV-DNA. Results: The first test detected the mothers of 219 cases of colostrum, 18 cases of HBV-DNA positive. These 18 cases were taken at least 24 hours after breast milk testing, only 1 case detected HBV-DNA. Conclusion: HBV-DNA of breast-fed mothers with HBV maternal lactation after 24h was significantly lower than that of the earliest colostrum. Therefore, it is best to remove HBV carriers of colostrum lactation 24 hours before breastfeeding, can improve the safety of breastfeeding.