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目的探讨一种能稳定、高效地建立小鼠原位肝移植模型且能单人操作的手术改良方法。方法参照“双袖套”法,肝上下腔静脉采用连续缝合,门静脉、肝下下腔静脉采用袖套法,胆管用支架法吻合,完成小鼠原位肝肝移植70例。术后观察24h,1周和1个月受体存活率,肝功能及肝组织病理变化。并设立假手术组对照。结果受体术后24h,1周和1个月存活率分别为95.7%,90.9%和85.1%。术后1周ALT逐渐升高,1个月降至正常水平;术后ALP值逐渐增高;病理显示肝组织结构良好。结论该方法成活率高,稳定性好,重复性强,是建立小鼠原位肝移植模型的理想方法。
Objective To discuss a method of surgical improvement that can establish a stable and efficient model of orthotopic liver transplantation in mice and can operate alone. Methods According to the “double sleeve” method, the superior and inferior hepatic vein was stitched continuously, the portal vein and inferior hepatic inferior vena cava were closed by cuff method and the bile duct was anastomosed by stent method. 70 cases of orthotopic liver transplantation in mice were completed. Postoperative 24h, 1 week and 1 month survival rate of the receptor, liver function and pathological changes of liver tissue. And set up sham group control. Results The survival rates of recipients at 24 h, 1 week and 1 month after operation were 95.7%, 90.9% and 85.1% respectively. The ALT increased gradually 1 week after operation and dropped to normal level one month later. ALP increased gradually after operation. The pathological examination showed that the structure of liver was good. Conclusion The method has the advantages of high survival rate, good stability and strong repeatability. It is an ideal method to establish a orthotopic liver transplantation model in mice.