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采用水蒸气蒸馏的方法,对采自云南4个不同地区(昆明,丽江,普洱,新平)的臭灵丹叶子进行挥发油提取,并利用GC-MS和GC-FID气相色谱联用技术分析其化学组成,分别从中鉴定出39、37、33、44个化合物。鉴定的化合物各占挥发油总量的93.32%(昆明)、80.57%(丽江)、92.24%(普洱)和93.85%(新平)。研究结果表明,采自这四个地区植物样品提取所得挥发油化学组成不尽相同:昆明产臭灵丹挥发油富含芳香族化合物(38.06%);而从丽江和普洱样品中获得的挥发油化学成分以倍半萜(分别为19.89%和44.65%)和氧化倍半萜(分别为40.85%和35.11%)为主;非氧化单萜和非氧化倍半萜化合物(分别为42.23%和33.25%)则是新平产臭灵丹挥发油的主要化合物类型。同时,对这些不同产地的臭灵丹挥发油也进行了抗菌活性测试。从以上实验研究结果推测环境差异是影响臭灵丹挥发油化学组成差异的重要因素,而不同的化学组成可能是导致4种样品抗菌敏感程度不同的原因。
Volatile oil was extracted from four different areas (Kunming, Lijiang, Pu’er, Xinping) by using steam distillation. The chemical constituents were analyzed by GC-MS and GC-FID gas chromatography From which 39, 37, 33, 44 compounds were identified respectively. The identified compounds accounted for 93.32% (Kunming), 80.57% (Lijiang), 92.24% (Pu’er) and 93.85% (Xinping), respectively, of the total volatile oil. The results showed that the chemical constituents of the essential oil extracted from the plant samples in these four regions were different: the volatile oil from the genus Kunming was rich in aromatic compounds (38.06%); while the chemical constituents from the samples from Lijiang and Pu’er were Sesquiterpenes (19.89% and 44.65%, respectively) and oxidized sesquiterpenes (40.85% and 35.11%, respectively). Non-oxidative monoterpenes and non-oxidated sesquiterpenoids (42.23% and 33.25%, respectively) It is the main compound type of the volatile oil from the new flat-bottomed plant. At the same time, the volatile oil from these different areas was also tested for antibacterial activity. According to the above experimental results, it is speculated that the environmental difference is an important factor that affects the chemical composition of Volatile Oil. However, different chemical compositions may be responsible for the different antibacterial sensitivity of the four samples.