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CD3AK细胞和LAK细胞均为异质细胞群，为了比较妇科恶性肿瘤患者LAK细胞和CD3AK细胞在体外培养过程中的表型特征，采用间接免疫荧光法动态观察CD3AK细胞和LAK细胞的CD_3~＋，CD_4~＋，CD_8~＋细胞率。结果表明：①培养第1周，CD3AK细胞的CD_3~＋，CD_4~＋，CD_8~＋细胞率分别为61．36％，54．23％和52．96％，显著高于LAK细胞组（P＜0．01）；③培养第2，3周，CD3AK细胞的CD_3~＋，CD_4~＋和CD_8~＋细胞率高于LAK细胞，但无统计学差异（P＞0．05）；③随着培养时间延长，CD3AK细胞CD_3~＋，CD_4~＋，CD_8~＋细胞率均不同程度下降，尤以CD_3~＋细胞率下降最快，而LAK细胞在培养第2周，CD_3~＋，CD_4~＋，CD_8~＋细胞率均有所升高，但第3周时又开始下降；④CD3AK细胞的CD_3~＋，CD_4~＋和CD_8~＋细胞率在培养第1周最高，而LAK细胞在培养第2周最高。提示：CD3MAb和IL－2激活CD3AK细胞和LAK细胞的途径不同，致使CD3AK细胞和LAK细胞向不同的表型特征分化。
In order to compare the phenotypic characteristics of LAK cells and CD3AK cells in gynecological malignancies in vitro, the CD3AK cells and LAK cells were heterogeneous. CD3AK cells and CD3AK cells were dynamically observed by indirect immunofluorescence staining. CD_4 ~ +, CD_8 ~ + cell rate. The results showed that: (1) At the first week of culture, the percentage of CD3 +, CD4 + and CD8 + cells in CD3AK cells was 61.36%, 54.23% and 52.96%, respectively, which was significantly higher than that in LAK cells <0.01) .③The percentage of CD3 +, CD4 + and CD8 + cells in CD3AK cells was higher than that in LAK cells at the second and third week of culture, but there was no significant difference (P> 0.05) With the prolongation of culture time, the percentage of CD_3 ~ +, CD_4 ~ + and CD_8 ~ + cells in CD3AK cells decreased to different extents, especially in CD3 + cells, while in LAK cells, the percentage of CD_3 ~ +, CD_4 ~ ~ +, CD_8 ~ + cells increased, but began to decline again at the third week; ④CD3AK cells had the highest rates of CD_3 ~ +, CD_4 ~ + and CD_8 ~ + cells in the first week, while LAK cells in the The highest in the second week of culture. Tip: CD3 MAb and IL-2 activate CD3AK cells and LAK cells in different ways, resulting in differentiation of CD3AK cells and LAK cells to different phenotypic characteristics.