Influence of green tea polyphenols on mitochondrial permeabi lity transition pore and Ca2+ transport

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The authors investigated the influence of green tea polyphenols (GTPs) on the liver mitochondria permeability transition pore (PTP) opening through mit ochondria swelling and change of mitochondria membrane potential. The data showe d that GTPs had obvious protective effect on the Ca2+-induced PTP opening in a d ose-dependent manner detected by mitochondria swelling. The results were obtain ed by measuring the change of mitochondria membrane potential through Rh 123. Further experiments were conducted to examine the detailed influence of GTP s on Ca2+import and export of mitochondria. The results showed that GTPs h ad rem arkably inhibitory effect on the Ca2+-induced Ca2+ import in mitoch ondria; and t hat they could accelerate Ca2+-release from mitochondria. Our data provid e an al ternate interpretation of the potent protective function of GTPs on cell against apoptosis.
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