论文部分内容阅读
为探讨脂多糖(LPS)及其受体TLR4相互作用并影响蜕膜NK细胞的可能机制,在孕E6.5给BALB/c×C57BL/6孕鼠腹腔注射LPS,而E10.5采用流式细胞术检测小鼠蜕膜NKG2D+TGF-β-NK细胞构成比,计算小鼠胚胎吸收率。此外,采用磁珠亲和细胞分选术纯化E10.5小鼠蜕膜NK细胞并培养,用LPS刺激所培养的细胞,并观察刺激后NK细胞表达NKG2D水平的变化。研究发现,在体内和体外实验中LPS刺激均可显著增高BALB/c×C57BL/6孕鼠蜕膜NK细胞NKG2D的表达水平,其中腹腔注射可显著增高胚胎吸收率。在体外细胞培养实验中,预先在培养基中加入抗TLR4抗体,则LPS刺激后细胞NKG2D表达水平无显著升高。这些结果提示,LPS与TLR4相互作用可增强小鼠蜕膜NK细胞NKG2D的表达,而NKG2D的过高表达不利于同种异基因妊娠成功。
To investigate the possible mechanism of the interaction between lipopolysaccharide (LPS) and its receptor TLR4 and affecting decidual NK cells, BALB / c × C57BL / 6 pregnant mice were injected intraperitoneally with LPS at E6.5, The percentage of NKG2D + TGF-β-NK cells in the decidua of mice was measured by cytometry, and the rate of embryo absorption in mice was calculated. In addition, E10.5 mouse decidual NK cells were purified by magnetic bead affinity cell sorting and cultured. The cultured cells were stimulated with LPS, and the changes of NKG2D expression in NK cells after stimulation were observed. The study found that in vivo and in vitro experiments LPS stimulation can significantly increase BALB / c × C57BL / 6 pregnancy decidual NK cells NKG2D expression levels, which intraperitoneally injected can significantly increase the rate of embryonic absorption. In vitro cell culture experiments, pre-added to the medium anti-TLR4 antibody, then LPS stimulation of cell NKG2D expression was no significant increase. These results suggest that the interaction between LPS and TLR4 can enhance the NKG2D expression in mouse decidual NK cells, while the overexpression of NKG2D is not conducive to the success of allogeneic pregnancy.