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目的:探讨HLA-G非翻译区多态性与重度子痫前期的发病有无相关性。方法:对孕晚期重度子痫前期患者、正常足月妊娠妇女,提取全血基因组DNA。利用PCR方法检测HLA-G3’UTR 14bp基因多态性,比较两组间等位基因及基因型的频率分布;利用巢氏PCR方法扩增HLA-G 5’URR上游调控区,并进行基因测序,比较两组间等位基因及基因型的频率分布。结果:①重度子痫前期组HLA-G 14bp缺失多态性的等位基因频率、基因型频率分布与正常妊娠组比较均无统计学差异(P>0.05);②HLA-G5’URR-716和-964位点基因型和等位基因型频率分布在重度子痫前期组和正常妊娠组间存在统计学差异(P<0.05);③HLA-G 5’URR-1140位点基因型和3’UTR 14bp多态性结合,在重度子痫前期组和正常妊娠组间存在统计学差异(P<0.05)。结论:①HLA-G 14bp缺失多态性的存在可减低母亲重度子痫前期的发病风险;②HLA-G 5’URR部分SNP可能与重度子痫前期的易感性相关;③HLA-G5’URR-1140位点基因型和HLA-G3’UTR 14bp多态性的结合可能与重度子痫前期的易感性有关。
Objective: To investigate the relationship between HLA-G untranslated region polymorphism and severe preeclampsia. Methods: Whole-blood genomic DNA was extracted from patients with severe preeclampsia and normal term pregnant women in the third trimester of pregnancy. The polymorphism of HLA-G3’UTR 14bp gene was detected by PCR and the allele and genotype frequencies were compared between the two groups. The upstream region of HLA-G 5’URR was amplified by nested PCR and sequenced , Comparing the frequency distribution of alleles and genotypes between the two groups. Results: ① There was no significant difference in HLA-G 14bp polymorphism between severe preeclampsia group and normal pregnancy group (P> 0.05). ② The frequencies of HLA-G5’URR-716 and The genotype and allele frequencies of -964 loci were significantly different between severe preeclampsia group and normal pregnancy group (P <0.05). ③ The genotypes of HLA-G 5’URR-1140 locus and 3’UTR 14bp polymorphism, there was a significant difference between severe preeclampsia group and normal pregnancy group (P <0.05). Conclusion: ① The presence of HLA-G 14bp polymorphism can reduce the risk of developing maternal severe preeclampsia. ② Partial SNP of 5’RRH-G may be associated with the susceptibility to severe preeclampsia. ③HLA-G5’URR-1140 The combination of point genotype and HLA-G3’UTR 14bp polymorphism may be related to the susceptibility to severe preeclampsia.