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本文报道一种快速、封片染色法,只需在60～64℃染色3～7分钟,比历时45～60分钟的微丝蚴常规染色法省时得多。先制备苏木素溶液。将1克苏木素粉和0.5克硫酸铁铵溶于100毫升45％的醋酸。再制备Hoyer封片液,将30克阿拉伯胶和20克氯醛合水溶于50毫升蒸馏水。将1份苏木素溶液和1份Hoyer封片液混合,每20毫升的混合液中加0.5毫升甘油,即得封片染液,最好放置10天后使用。
This paper reports a rapid, patch-based staining method that requires only staining at 60-64 ° C for 3-7 minutes and is much more time-consuming than conventional staining of microfilariae over a period of 45-60 minutes. Hematoxylin solution was prepared first. One gram of hematoxylin and 0.5 gram of ammonium ferric sulfate were dissolved in 100 mL of 45% acetic acid. A further Hoyer lidding solution was prepared, dissolving 30 grams of gum arabic and 20 grams of chloral hydrate in 50 ml of distilled water. One part of hematoxylin solution and 1 part of Hoyer packing liquid mixture, 20 ml of liquid mixture to add 0.5 ml of glycerol, the seal dye, the best place for 10 days before use.