论文部分内容阅读
目的：研究汉族GGCX基因rs11676382位点多态性与心房颤动（AF）的相关性。方法以200例汉族未服用华法林的心房颤动患者作为研究组，200例健康汉族患者作为对照组，所有患者均采集外周血提取基因组 DNA，采用聚合酶链反应，限制性片段长度多态性技术（PCRRFLP ）检查患者 GGCX 基因rs11676382多态性的基因型和等位基因频率分布，观察GGCX基因rs11676382多态性与心房颤动的关系。结果 GGCX基因rs11676382位点在入选人群中的基因型及等位基因分别为CC、CG和GG型，其基因型频率在心房颤动组和对照组分别依次为82．5％、16．5％、1．0％和85．0％、0．5％、14．5％，分布趋势相同，差异无统计学意义（P＞0．05）；C和G等位基因频率在心房颤动组和对照组分别为99．0％，1．0％和99．5％，0．5％，C和G等位基因频率在两组间的差异无统计学意义（P＞0．05）。结论 GGCX基因rs11676382多态性与未服用华法林的心房颤动无相关性。“,”Objective To investigate the Correlation between the rs1 1676382 polymorphism of GGCX gene with atrial fibrillation (AF)in Han Chinese in Xinjiang.Methods There were 200 Han Chinese patients who not taking warfarin with atrial fibrillation were collected as study group,and 200 healthy cases were collected as control group.The DNA of peripheral venous blood was extracted.The rs1 1676382 polymorphism of GGCX gene were detected by polymerase chain reaction-restriction enzyme fragment length polymorphism (PCR-RFLP).The GGCX genotype and allele distribution were analyzed.Result The GGCX genotype were CC,CG and GG,and the genetype frequency in the study group was 82.5%,16.5% and 1%,and 15.85%,14.5%,0.5% in the control group.There were no significant differences in the genotype distribution of Rs1 1676382 GGCX gene loci in the Han Chinese between the study group and the control group (P>0.05);The allele C and G frequencies were 99%and1%in the study group, 99.5% and 0.5%,in the control group.There were no statistical significance in the allele C and G frequencies be-tween the two groups(P>0.05 ).Conclusion There are no correlation between the GGCX gene rs1 1676382 poly-morphism and atrial fibrillation in the patients who were not taking warfarin.