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目的比较经长途运输后的滤白悬浮红细胞质量指标的差异。方法选取从云南省地州血站采集并检测合格的悬浮红细胞(1.5 U)200份,留取血液标本作为对照组Ⅰ,这200份悬浮红细胞在当地滤白处理后,经8-10h的长途运输送达本院输血科,再留取此200份作为检测组Ⅰ;同时选取从云南省地州血站同一时间采集并检测合格的,同一批次悬浮红细胞(1.5 U)160份,留取血液标本作为对照组Ⅱ,这160份悬浮红细胞与Ⅰ组一起经8-10 h长途运输送达本院,在输血科滤白处理后,留取标本作为检测组Ⅱ。分别检测这4组血液标本的游离血红蛋白(FHb)、红细胞比容(Hct)、总血红蛋白(Hb)、K+、乳酸脱氢酶和红细胞变形指数(EI),计算各组红细胞溶血率,并进行各检测指标的对比分析。结果检测组Ⅰ与对照组Ⅰ比较,红细胞溶血率明显上升(P<0.05),K+、乳酸脱氢酶上升(P<0.05),EI下降(P<0.05);检测组Ⅱ与对照组Ⅱ比较,红细胞溶血率、K+、乳酸脱氢酶及EI无明显变化(P>0.05)。结论未滤白的悬浮红细胞经长途运输后,输注前再进行滤白的红细胞检测质量相对较好,为保障临床输血安全,经长途运输血液时,应尽量选用未滤白的悬浮红细胞。
Objective To compare the difference of quality indexes of leucocytes after long-haul transport. Methods 200 cases of qualified erythrocytes (1.5 U) were collected and tested from Daczhou Blood Station of Yunnan Province. The blood samples were taken as control group Ⅰ. After the 200 red blood cells were filtered out locally, the long-distance 8-10h Transport to reach our hospital blood transfusion department, and then take the 200 as a test group Ⅰ; at the same time selected from prefectural Yunnan Blood Station collected and tested at the same time, the same batch of 160 units of suspended red blood cells (1.5 U) Blood samples were taken as control group Ⅱ. These 160 suspended erythrocytes were delivered to our hospital together with group Ⅰ by long-distance transportation for 8-10 h. After filtration treatment in blood transfusion department, samples were taken as test group Ⅱ. The free hemoglobin (FHb), hematocrit (Hct), total hemoglobin (Hb), K +, lactate dehydrogenase and erythrocyte deformability index (EI) were detected in the four groups of blood samples. Comparative analysis of each test index. Results Compared with control group Ⅰ, the hemolysis rate of erythrocytes was significantly increased (P <0.05), K +, lactate dehydrogenase (P <0.05) and EI decreased (P <0.05). Compared with control group Ⅱ , Erythrocyte hemolysis rate, K +, lactate dehydrogenase and EI had no significant change (P> 0.05). Conclusion After the long-distance transport of unfiltered suspended erythrocytes, the quality of red blood cells before filtration is relatively good. In order to ensure the safety of clinical blood transfusion, the unfiltered white blood cells should be used as far as possible when transporting blood.