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以光增白剂M2R作为影响因子,探讨其对草地螟Loxostege sticticalis幼虫围食膜(peritrophic membrane,PM)的作用机理。通过环境扫描电镜观察和生化测定研究了光增白剂对草地螟幼虫围食膜结构和蛋白质种类的影响,及其对Bt毒力的增效作用。结果表明:围食膜含有多种蛋白质,经SDS-PAGE测定至少有19条带,分子量在94kD以下,幼虫取食光增白剂可影响围食膜中几丁质结合蛋白(chitin binding proteins,CBPs)的含量。不同浓度的光增白剂可以对草地螟围食膜的形态结构产生明显的影响,正常的围食膜表面光滑致密、无孔洞和缝隙,增白剂处理的围食膜产生了孔缝。生测实验表明,添加光增白剂后能够显著缩短Bt的杀虫时间,降低Bt的使用浓度。可见,光增白剂可对草地螟围食膜产生损伤,进而提高了Bt的防治效果。
Using optical brightener M2R as the influencing factor, the mechanism of action on the peritrophic membrane (PM) of larvae of Loxostege sticticalis was discussed. The effects of optical brighteners on the peritrophic membrane structure and protein type of larvae of S. furnacalis and their synergistic effects on the virulence of Bt were studied by environmental scanning electron microscopy and biochemical assays. The results showed that there were at least 19 bands detected by SDS-PAGE and the molecular weight was below 94 kD. The larvae fed optical brighteners could affect the expression of chitin binding proteins CBPs) content. Different concentrations of optical brighteners can have a significant impact on the morphological structure of the peritrophic membrane. The normal peri-merous membrane surface is smooth and dense, without holes and gaps, and the peri-membrane perforation produced by the whitening agent has a pore slit. Bioassay experiments show that adding optical brightener can significantly shorten the insecticidal time of Bt, reduce the use of Bt concentration. Visible, optical brightener on the grasshopper peritoneal membrane damage, thereby increasing the prevention and treatment of Bt.