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目的：探讨氧自由基在家兔低血容量性休克再灌注损伤中的作用，并观察川芎嗪对其有无保护效应。方法：制备家兔低血容量性休克模型，检测血浆和组织超氧化物歧化酶（ＳＯＤ）活性及丙二醛（ＭＤＡ）含量。实验分为川芎嗪保护组和未用川芎嗪的非保护组，并进行比较。结果：休克前两组动物ＳＯＤ活性及ＭＤＡ值均无统计学差异。休克９０分钟时两组动物ＳＯＤ活性均显著下降（川芎嗪保护组为１８７±３６ＮＵ／ｍｌ，非保护组为１８９±３７ＮＵ／ｍｌ），ＭＤＡ值均显著升高（保护组８．１０±０．９０μｍｏｌ／Ｌ，非保护组８．１０±０．８８μｍｏｌ／Ｌ）；休克再灌注后，保护组ＳＯＤ活性逐渐上升，休克再灌注３小时后接近休克前水平（３４２±２９ＮＵ／ｍｌ），明显高于休克９０分钟（１８７±３６ＮＵ／ｍｌ）和非保护组同时相水平（１２１±２２ＮＵ／ｍｌ）；保护组ＭＤＡ逐渐下降，休克再灌注３小时后（６．００±０．６２μｍｏｌ／Ｌ）明显低于休克９０分钟时（８．１０±０．９０μｍｏｌ／Ｌ）及非保护组同时相水平（９．３０±０．９６μｍｏｌ／Ｌ）。此外，川芎嗪保护组心、肝、肾、肠道组织ＳＯＤ活性明显高于非保护组ＳＯＤ活性；心、肝、肠道ＭＤＡ含量明显低于非保护组ＭＤＡ值。?
Objective: To investigate the role of oxygen free radicals in hypovolemic shock-reperfusion injury in rabbits and to observe whether tetramethylpyrazine has protective effect on it. Methods: Rabbit model of hypovolemic shock was prepared. The activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in plasma and tissue were measured. The experiment was divided into tetramethylpyrazine group and non-tetramethylpyrazine group, and compared. Results: There was no significant difference in SOD activity and MDA between the two groups before shock. At 90 minutes of shock, the activity of SOD in both groups was significantly decreased (187 ± 36 NU / ml in the tetramethylpyrazine group and 189 ± 37 NU / ml in the non-protective group), MDA values were significantly increased (protective group 8.10 ± 0. 90 micromol / L, and 8.10 ± 0.88 micromol / L in non-protective group). After shock-reperfusion, the activity of SOD in protective group increased gradually and reached the level of 342 ± 29NU / ml 3h after shock reperfusion At the same time, the levels of MDA in protective group decreased gradually at 90 min (187 ± 36 NU / ml) and 121 ± 22 NU / ml in non-protected group (6.00 ± 0.62 μmol / L) 3 h after shock reperfusion Lower than that of shock at 90 minutes (8.10 ± 0.90μmol / L) and the non-protective group at the same time (9.30 ± 0.96μmol / L). In addition, the SOD activity in the heart, liver, kidney and intestine of the tetramethylpyrazine group was significantly higher than that of the non-protective group. The content of MDA in heart, liver and intestine was significantly lower than that in the non-protective group. ?