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目的:探讨婴幼儿血管瘤增生及凋亡的过程,为临床治疗提供参考。方法:选取术前未经治疗的38例血管瘤手术切除标本,采用免疫组化增殖细胞核抗原（PCNA）及末端脱氧核苷酸转移酶介导的脱氧尿苷三磷酸切口末端标记法（TUNEL）,检测其PCNA指数及凋亡指数（AI）。结果:增生期血管瘤婴幼儿的平均年龄为（4.6±3.0）个月,PCNA指数为3.27±0.60,AI为1.69±0.96;退化期血管瘤婴幼儿的平均年龄为（17.0±7.2）个月,PCNA指数为1.39±0.50,AI为26±13.93,两组差异有显著性。结论:血管内皮细胞增生及凋亡存在于婴幼儿血管瘤的整个自然过程中:在增生期,其血管内皮细胞的增殖活性大于凋亡的活性,血管瘤呈增大趋势;在退化期,其血管内皮细胞的凋亡活性大于增殖的活性,血管瘤呈缩小趋势。
Objective: To investigate the process of hyperplasia and apoptosis of infantile hemangioma and provide reference for clinical treatment. Methods: Thirty - eight cases of hemangiomas without operation were selected for surgical resection. The expressions of proliferating cell nuclear antigen (PCNA) and terminal deoxynucleotidyl transferase - mediated deoxyuridine triphosphate nick end labeling (TUNEL) , Detected PCNA index and apoptosis index (AI). Results: The mean age of infants with proliferative hemangiomas was (4.6 ± 3.0) months, PCNA index was 3.27 ± 0.60, AI was 1.69 ± 0.96, and the average age of infantile hemangiomas was (17.0 ± 7.2) months , PCNA index was 1.39 ± 0.50, AI was 26 ± 13.93, there was significant difference between the two groups. CONCLUSION: Vascular endothelial cell proliferation and apoptosis exist in the whole process of infantile hemangiomas. In the proliferative phase, the proliferation activity of vascular endothelial cells is greater than that of apoptosis, and the hemangiomas show an increasing trend. During the degradation period, Vascular endothelial cell apoptosis activity is greater than the proliferation of activity, hemangioma shrinking trend.