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目的为尿液中敌草快的测定研究一种简单可靠的检测方法。方法将尿样高速离心,上清液转移至试管中,加入不同的碱性溶液调节p H,加入连二亚硫酸钠显色,于378nm比色测定。结果敌草快在0.1~10μg/m L呈良好线性,相关系数为0.998 8,回归直线方程Y=0.114 9X+0.010 3。最低检出限为0.02μg/m L,日间和日内精密度分别为4.50%,6.28%。结论此方法操作简便、快速,适用于临床监测和法医毒物分析。
The purpose for the determination of diuretics in urine fast A simple and reliable detection method. Methods The urine sample was centrifuged at high speed and the supernatant was transferred to a test tube. Different basic solutions were added to adjust p H, and sodium dithionite was added to develop a colorimetric assay at 378 nm. Results Diuron was linear at 0.1-10 μg / mL, with a correlation coefficient of 0.998 8. The regression equation was Y = 0.114 9X + 0.010 3. The lowest detection limit was 0.02μg / m L, the intra-day and intra-day precision were 4.50% and 6.28% respectively. Conclusion This method is simple, rapid and suitable for clinical monitoring and forensic toxicology analysis.