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目的:观察疏肝健脾方药对多囊卵巢综合征大鼠性激素水平及胰岛素抵抗作用。方法:随机将60只健康雌性SD大鼠分为正常组、模型组、中药低剂量组[22.05g/(kg·d)~(-1)]、中药中剂量组[44.1g/(kg·d)~(-1)]和中药高剂量组[88.2 g/(kg·d)~(-1)],采用脱氢表雄酮(DHEA)皮下注射进行PCOS模型复制。造模成功后,连续给药21 d后,分离血清,检测大鼠血清T、E2、LH、FSH、FBG,FINS水平并计算HOMA-IR水平,分离大鼠卵巢组织,RT-PCR法检测GSK3β、AKT2、GLUT4、IRS-1 m RNA水平。结果:与正常组比较,模型组大鼠血清T、LH、FSH、FBG、FINS及HOMA-IR水平显著升高(P<0.01),E2、P水平显著下降(P<0.01),卵巢组织中GSK3β、AKT2、GLUT4、IRS-1 m RNA水平显著降低(P<0.01);经过治疗后,与模型组比较,低剂量组大鼠血清E2、P显著升高(P<0.05,P<0.01),LH、FBG、FINS水平降低(P<0.05),HOMA-IR水平显著降低(P<0.01),卵巢组织中GSK3β、IRS-1m RNA水平显著升高(P<0.05);中剂量组和高剂量组大鼠血清T、LH、FSH、FBG、FINS及HOMA-IR水平均显著降低(P<0.01,P<0.05),E2、P水平显著升高(P<0.01),卵巢组织中GSK3β、AKT2、GLUT4、IRS-1 m RNA水平均显著升高(P<0.01)。结论:疏肝健脾方药能够有效改善PCOS大鼠血清激素水平及胰岛素抵抗情况,其机制可能与调控PCOS大鼠卵巢组织中GSK3β、AKT2、GLUT4、IRS-1 m RNA水平有关。
Objective: To observe the effect of Shugan Jianpi Prescription on sex hormones and insulin resistance in rats with polycystic ovary syndrome. Methods: Sixty healthy female SD rats were randomly divided into normal group, model group, low dose Chinese herbal group [22.05g / (kg · d) -1], middle dose group (44.1g / (kg · The rats were injected subcutaneously with dehydroepiandrosterone (DHEA) for replicating in PCOS model, and the high dose group (88.2 g / (kg · d) -1). After 21 days of continuous administration, the serum was separated and serum levels of T, E2, LH, FSH, FBG and FINS were measured and HOMA-IR levels were calculated. Ovariectomized rats were isolated and the expression of GSK3β , AKT2, GLUT4, IRS-1 m RNA levels. Results: Compared with the normal group, the levels of serum T, LH, FSH, FBG, FINS and HOMA-IR in model group were significantly increased (P <0.01) The levels of GSK3β, AKT2, GLUT4 and IRS-1 mRNA were significantly decreased (P <0.01). After treatment, the levels of E2 and P in low dose group were significantly increased (P <0.05, P <0.01) , LH, FBG and FINS (P <0.05), HOMA-IR level decreased significantly (P <0.01), and levels of GSK3β and IRS-1mRNA in ovary tissue increased significantly The levels of serum T, LH, FSH, FBG, FINS and HOMA-IR in the dose group were significantly decreased (P <0.01, P <0.05) AKT2, GLUT4, IRS-1 m RNA levels were significantly increased (P <0.01). Conclusion: Shuganjianpi Decoction can effectively improve serum hormone levels and insulin resistance in PCOS rats. The mechanism may be related to the regulation of GSK3β, AKT2, GLUT4 and IRS-1 mRNA levels in ovarian tissue of PCOS rats.