Trauma and pulmonary thromboembolism:an experimenta study on their correlation

来源 :Chinese Journal of Traumatology | 被引量 : 0次 | 上传用户:larry_john
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Objective:To investigate the correlation between trauma and pulmonary thromboembolism. Methods:Comminuted fractures and extensive soft- tissue contusion at both hind limbs were made by a falling weight from a height in 16 rabbits.Lung perfusion scanning was performed to obtain the radioactivity counts before trauma,at 1 h,48 h and 96 h after trauma.All the data were divided into 4 groups based on the above 4 time points.The rabbits were sacrificed when positive findings on the pulmonary perfnsion scanning appeared.Their lungs were harvested to be paraffin-embedded and stained with hematoxylin-erosin method for histological examination of thromboembolism.The randomized block design ANOVA and the method of least significant difference(LSD)were used for statistical analysis of the radioactivity counts. Results:The histological findings showed that pulmonary embolism developed in 6 of the 16 rabbits (37.5%).Five of the 6 pulmonary embolism rabbits presented neither clinical symptoms nor positive pulmonary embolism manifestations in the lung perfusion scanning.A significant difference was found in lung perfusion radioactivity between the pre-traumatic,post-traumatic 1h groups and post-traumatic 48 h and 96 h groups(P<0. 05). Conclusions:Fractures of the hind limbs accompanied with extensive soft-tissue contusion may cause pulmonary micro-embolism that is not sensitive to lung perfusion scanning and tends to have no clinical symptoms. Pulmonary embolism development may take more than two days after trauma. Objective: To investigate the correlation between trauma and pulmonary thromboembolism. Methods: Comminuted fractures and extensive soft-tissue contusion at both hind limbs were made by a falling weight from a height in 16 rabbits. Lung perfusion scanning was performed to obtain the radioactivity counts before trauma, at 1 h, 48 h and after 96 h after trauma. All data were divided into 4 groups based on the above 4 time points. The rabbits were sacrificed when positive findings on the pulmonary perfusion scanning documented. Their lungs were harvested to be paraffin-embedded and stained with hematoxylin-erosin method for histological examination of thromboembolism. The randomized block design ANOVA and the method of least significant difference (LSD) were used for statistical analysis of the radioactivity counts. Results: The histological findings showed that pulmonary embolism Five in the 6 pulmonary embolism rabbits presented two clinical symptoms nor po sitive pulmonary embolism manifestations in the lung perfusion scanning. A significant difference was found in lung perfusion radioactivity between the pre-traumatic, post-traumatic 1h groups and post-traumatic 48 h and 96 h groups (P <0.05). Conclusions: Fractures of the hind limbs accompanied with extensive soft-tissue contusion may cause pulmonary micro-embolism that is not sensitive to lung perfusion scanning and tends to have no clinical symptoms. Pulmonary embolism development may take more than two days after trauma.
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