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背景:进一步验证辛夷挥发油所具有抗非特异性炎症的作用,为此实验的起因。目的:探讨辛夷挥发油对大鼠佐剂性关节炎的作用。设计:分层抽样,随机分组对照,量效分析。单位:成都中医药大学药学院药理教研室。材料:实验于1999-02/04在成都中医药大学药理实验室完成。选用Wistar大鼠120只,按体质量分层后随机分组。方法:Wistar大鼠分成4组:模型对照组,辛夷挥发油0.28mL/kg及0.14mL/kg组,地塞米松25mg/kg组。各组动物均在足跖部皮内注射完全弗氏佐剂(0.1mL/只)致敏,以诱发多发关节炎。依给药方案不同,实验分3批次进行,各个批次每组大鼠10只。第1批次在致敏前3d开始给动物灌药,连续给药3d,观测药物对佐剂引起的急性非特异性炎症的作用;第2批次在致敏后第8天开始给动物灌药,连续给药7d,观测药物对继发病变的预防作用;第3批次在致敏后第19天开始给动物灌药,连续给药7d,观测药物对继发病变的治疗作用,。测量大鼠足肿胀程度及距小腿(踝)关节组织中前列腺素E2含量。主要观察指标:①辛夷挥发油对佐剂引起的急性非特异性炎症的作用。②对佐剂性关节炎的预防作用。③对佐剂性关节炎的治疗作用。结果:①辛夷挥发油能极显著抑制原发性足肿胀。②预防给药时辛夷挥发油0.28mL/kg,0.14mL/kg组均能极显著地抑制继发性关节肿胀[(0.231±0.065)mL,(0.206±0.082)mL],减少肿胀组织中前列腺素E2含量[(0.1332±0.0572),(0.1385±0.0426)A278nm]。③治疗性给药时,辛夷挥发油0.14mL/kg组对继发性关节炎性肿胀无明显作用,但能显著减少肿胀组织中前列腺素E2含量;辛夷挥发油0.28mL/kg组对上述参数有极显著或显著作用。结论:辛夷挥发油对完全弗氏佐剂引起的原发性足肿胀和继发性关节炎都有抑制作用,预防作用优于治疗作用,治疗作用时大剂量组效果较好。
Background: Further verification of the anti-non-specific inflammation effect of Magnolia bark volatile oil was the cause of this experiment. Objective: To investigate the effect of volatile oil from Magnolia barbatae on adjuvant arthritis in rats. Design: Stratified sampling, randomized control, and dose-effect analysis. Unit: Department of Pharmacology, School of Pharmacy, Chengdu University of Traditional Chinese Medicine. MATERIALS: The experiment was performed at the Laboratory of Pharmacology, Chengdu University of Traditional Chinese Medicine from September to April 1999. 120 Wistar rats were selected and randomly divided into groups according to body mass. Methods: Wistar rats were divided into 4 groups: model control group, V. chinensis volatile oil 0.28mL/kg and 0.14mL/kg group, dexamethasone 25mg/kg group. All groups of animals were injected intradermally with complete Freund’s adjuvant (0.1 mL/body) to induce multiple arthritis. According to different dosing schedules, the experiment was divided into 3 batches. Each group had 10 rats in each batch. In the first batch, animals were given 3 days before sensitization, and continuous administration for 3 days observed the effects of the drug on acute non-specific inflammation caused by adjuvant; the second batch began to administer the animals on the 8th day after sensitization. Continuous administration for 7 days was used to observe the preventive effect of the drug on secondary lesions. The third batch was given to the animals on the 19th day after sensitization. Continuous administration for 7 days was performed to observe the therapeutic effect of the drug on secondary lesions. The degree of paw swelling in the rat and the content of prostaglandin E2 in the joint tissue of the calf were measured. MAIN OUTCOME MEASURES: The effect of volatile oil of V. mutton on acute non-specific inflammation caused by adjuvant. 2 The preventive effect of adjuvant arthritis. 3 Therapeutic effects on adjuvant arthritis. Results: 1 The volatile oil of V. chinensis significantly inhibited the swelling of the primary foot. 2 The 0.28 mL/kg M. chinense volatile oil at the time of prevention and administration, 0.14 mL/kg group can significantly inhibit secondary joint swelling [(0.231±0.065) mL, (0.206±0.082) mL], and reduce prostaglandin in swollen tissue. E2 content [(0.1332 ± 0.0572), (0.1385 ± 0.0426) A278 nm]. 3 When administered therapeutically, Magnolia barkiana volatile oil 0.14mL/kg group had no significant effect on secondary arthritis swelling, but it could significantly reduce the prostaglandin E2 content in the swollen tissue; the volatile oil 0.28mL/kg of Magnolia barbadensis had the above parameters. Significant or significant effect. Conclusion: M. magnoliae volatile oil has inhibitory effect on primary foot swelling and secondary arthritis caused by complete Freund’s adjuvant, and the preventive effect is better than the therapeutic effect. The effect of large dose group is better when the treatment effect.