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目的 探讨三氧化二砷(砒霜)或ATRA对原代APL细胞或HL-60细胞影响,同时分析三氧化二砷与ATRA之间的交叉耐药性。方法 MTT法测定HL-60细胞或APL细胞的增殖性。流式细胞仪检测两类细胞的凋亡性,NBT法用于测定HL-60细胞或原代APL细胞的分化性。结果 二氧化二砷与ATRA均能明显抑制APL细胞或HL-60细胞的体外增殖,它们作用机制分别为诱导明显的凋亡或分化。体外三氧化二砷对ATRA耐药HL-60细胞具有明显的抑制增殖作用,并诱导其凋亡发生。结论 三氧化二砷与ATRA无交叉耐药性。研究结果表明,ATRA诱导的高白细胞征并非归因于分化大于凋亡。
Objective To investigate the effects of arsenic trioxide (Arsenic trioxide) or ATRA on primary APL cells or HL-60 cells, and to analyze the cross-resistance between arsenic trioxide and ATRA. Methods The proliferation of HL-60 cells or APL cells was determined by MTT assay. Flow cytometry was used to detect the apoptotic properties of both types of cells, and NBT method was used to determine the differentiation of HL-60 cells or primary APL cells. Results Both arsenic trioxide and ATRA could significantly inhibit the proliferation of APL cells or HL-60 cells in vitro. Their mechanisms of action were induced apoptosis or differentiation, respectively. In vitro, arsenic trioxide has obvious antiproliferative effects on ATRA-resistant HL-60 cells and induces apoptosis. Conclusion There is no cross-resistance between arsenic trioxide and ATRA. The results of the study indicate that ATRA-induced high leukocyte signs are not due to differentiation greater than apoptosis.