论文部分内容阅读
目的：探讨支气管哮喘发病机制中Ｔ细胞的调节作用。方法：分离出病人辅助性Ｔ细胞中的幼稚性（Ｎａｉｖｅ）Ｔ细胞亚群即ＣＤ４＋ＣＤ４５ＲＡ＋Ｔ细胞亚群，并将其与各自的Ｂ淋巴细胞共同培养。同时设定非特异性刺激原ＰＷＭ（美洲商陆）刺激组及非刺激组，测定培养上清液中ＩｇＥ含量。结果：非刺激组病人ＩｇＥ含量明显高于健康对照（Ｐ＜００１），刺激组病人与健康对照ＩｇＥ含量无显著差别（Ｐ＞００５）。结论：支气管哮喘病人幼稚性Ｔ细胞在ＩｇＥ产生中起到了正向促进作用
Objective: To investigate the regulatory role of T cells in the pathogenesis of bronchial asthma. Methods: The naive T lymphocyte subsets, CD4 + CD45RA + T cell subsets, were isolated from patient-assisted T cells and co-cultured with their respective B lymphocytes. At the same time, the non-stimulated group and non-stimulated original PWM (non-stimulated) PWM were set up, and the content of IgE in the culture supernatant was determined. Results: IgE levels in non-stimulation group were significantly higher than those in healthy control group (P <001). There was no significant difference in IgE level between stimulation group and healthy control group (P> 005). Conclusions: The naive T cells from patients with bronchial asthma play a positive role in IgE production