论文部分内容阅读
1983年采用致瘤农杆菌(Agrobacterium tumefacies)的C58、T37、B3/73三个菌种,感染Beta Vulgaris和B.maritima二个种的106个品种和品系。在102个品种上诱导出冠瘿瘤。其中,B.Vulgaris品种和一个野生杂种诱导出畸胎瘤。为进一步验证诱导畸胎瘤的重演性及形成条件,今年又设置了重复试验,实验结果,再一次形成了畸胎瘤(图1—2)。经过对畸胎瘤幼苗叶片生化检测,叶片中含有胭脂碱(图3)。证明胭碱合成酶基因,在细胞再生过程中仍然保存在再生植株中。畸胎瘤的重复出现,为解决含T—DNA细胞系的再生,开展甜菜基因工程的研究,奠定了良好的基础。
In 1983, three strains C58, T37 and B3 / 73 of Agrobacterium tumefacies were used to infect 106 varieties and strains of two species of Beta Vulgaris and B. maritima. Tumor was induced on 102 cultivars. Among them, B. vulgaris and one wild hybrid induced teratoma. In order to further verify the teratogenicity of teratoma and the conditions for the formation of repeated set of tests this year, the experimental results, and once again formed a teratoma (Figure 1-2). After teratocarcinoma seedling leaf biochemical tests, the leaves contain nopaline (Figure 3). Prove nostalgic synthase gene, in the cell regeneration process is still preserved in regenerated plants. Teratoma recurrence, in order to solve the regeneration of T-DNA containing cell lines, to carry out the genetic engineering of sugar beet, and laid a good foundation.