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目的采用3种不同复性方法获得具有生物活性的重组蛋白蓝氏贾第鞭毛虫(Giardia lamblia,Gl)沉默信息调节因子2(GlSir2),并比较3种方法的复性效率,为研究其活性和生物学功能奠定基础。方法表达并纯化GlSir2的包涵体,分别采用稀释复性、透析复性和柱上复性等3种方法对GlSir2包涵体进行复性,并通过检测复性蛋白的活性,比较3种复性方法的特点。结果蛋白活性回收以柱上复性较高,为1 712Flu,透析复性和稀释复性法分别为758Flu和206Flu。结论 3种复性方法均能获得有活性的融合蛋白GlSir2,其中以柱上复性方法处理的蛋白活性回收值最高。
OBJECTIVE: To obtain the biologically active recombinant protein Gljir2 of Giardia lamblia (Gl) using three different refolding methods and to compare the refolding efficiency of the three methods. And biological function laid the foundation. Methods The inclusion bodies of GlSir2 were expressed and purified. GlSir2 inclusion bodies were refolded by three methods: dilution refolding, dialysis refolding and column refolding, respectively. The refolding activities of the GlSir2 inclusion bodies were also tested. The three refolding methods specialty. Results The activity of protein was recovered to a high value of 1 712Flu on the column, and the refolding and dilution refolding methods were 758Flu and 206Flu, respectively. Conclusion All of the three refolding methods can obtain the active fusion protein GlSir2, of which the activity of the protein was the highest on the column.