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Objective:The aim of this study is to evaluate the effect of CO2 pneumoper-itoneum on growth and spread of intraperitoneal tumor in an animal model.Methods:We estab-lished an animal model of epithelial ovarian cancer in immunocompetent rat Fischer 344.Twen-ty rats were randomized to two groups(10rats/group):CO2 insufflation and sham laparotomv.Tumors were excised from the rats in each group 28 days after operation.Ascites,tumor mass.local regional invasion incidence,lymph node involvement.and liver and lung metastases were evaluated.Sections of tumors were made and then the cell cycle fraction were measured by quantitating DNA in individual cells using flow cytometry analysis.The expressions of Ki-67.VEGF,and CD44v6 protein were determined using immunocytochemistry bv flow cytometry.Re-suits:Tumor mass,local regional invasion incidence and the amount of ascites were higher in CO2 insufflation than those in laparotomy,but there were no significant differences between two groups.The expression of CDd4v6 protein was higher in CO2 insufflation than that in laparotomv (P=0.002).There was no significant difference between two groups in the cells cvcle fraction and the expressiones of Ki-67 and VEGF protein.Conclusions:CO2 pneumoperitoneum has effects on intraperitoneal tumor growth and metastasis in the animal model.