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进行大豆蛋白质含量相关的QTL定位可为分子标记辅助选择(MAS)育种和基因挖掘提供依据。本研究选用综合性状优良、蛋白质含量高的中黄13作轮回亲本,低蛋白质含量的东山69作供体亲本,构建了BC2F2、BC2F3回交导入系群体,采用SSR分子标记技术,获得含86个SSR标记、覆盖1 400.1cM、由19个连锁群组成的遗传连锁图谱a,利用完备区间作图法(ICIM),对BC2F2、BC2F3分离群体的蛋白质含量进行QTL分析。结果表明:在BC2F2家系中检测到3个蛋白质含量相关QTL,分布于A1、C1和J连锁群,表型贡献率分别为10.00%、7.07%和9.23%;在BC2F3家系中检测到4个蛋白含量相关QTL,分布于B1、C1、I和J连锁群,表型贡献率分别为17.57%、3.46%、8.53%和11.80%。标记区间Satt396~Satt180和Sct_001~Satt654在BC2F2、BC2F3家系中被同时检测到,且Sct_001~Satt654内发现的QTL很可能是一个与蛋白质含量相关的新位点。
QTL mapping related to soybean protein content may provide a basis for molecular marker-assisted selection (MAS) breeding and gene mining. In this study, we selected Zhonghuan 13 as the recurrent parent and low-protein content Dongshan 69 as donor parent with good trait and high protein content to construct BC2F2 and BC2F3 backcross inbred lines. SSR molecular markers were used to obtain 86 SSR marker covering a genetic linkage map consisting of 19 linkage groups at 1 400.1 cM. A. QTL analysis of the protein content of BC2F2 and BC2F3 segregating populations using complete interval mapping (ICIM). The results showed that three QTLs related to protein content were detected in BC2F2 pedigrees distributed in A1, C1 and J linkage groups, with the phenotypic contribution rates of 10.00%, 7.07% and 9.23%, respectively. Four proteins were detected in the BC2F3 pedigree QTLs distributed in B1, C1, I and J linkage groups, the phenotypic contribution rates were 17.57%, 3.46%, 8.53% and 11.80%, respectively. The marker interval Satt396 ~ Satt180 and Sct_001 ~ Satt654 were detected in both BC2F2 and BC2F3 pedigrees, and the QTLs found in Sct_001 ~ Satt654 are likely to be a new site related to protein content.