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目的 探讨慢性乙肝患者血清细胞因子 (TGF -β1、TNF -α、IFN -γ)在肝纤维化中的作用机制。 方法 采用ELISA和RIA方法检测 95例慢性乙肝患者血清TGF -β1、TNF -α、IFN -γ、HA、PCⅢ、C -Ⅳ的水平 ,并与肝组织病理学变化进行对照研究。 结果 慢性乙肝患者血清TGF -β1、TNF -α、HA、PCⅢ、C -Ⅳ的含量均不同程度高于对照 ,且随肝损害程度的加重而升高 ,与肝损害程度呈正相关 ;并且其水平与肝纤维化程度具有明显的相关性 (P <0 0 5或P <0 0 1)。血清TGF -β1水平与血清HA、PCⅢ、C -Ⅳ水平具有直线相关性 ,而IFN -γ与血清HA、PCⅢ、C -Ⅳ水平呈负相关。结论 慢性乙肝患者血清TGF -β1、TNF -α、IFN -γ水平与肝纤维化相关 ,其血清含量可以作为诊断肝纤维化的血清学指标
Objective To investigate the mechanism of serum cytokines (TGF-β1, TNF-α and IFN-γ) in the pathogenesis of hepatic fibrosis in chronic hepatitis B patients. Methods Serum levels of TGF-β1, TNF-α, IFN-γ, HA, PCⅢ and C-Ⅳ in 95 patients with chronic hepatitis B were detected by ELISA and RIA, and compared with the pathological changes of liver tissue. Results The levels of serum TGF-β1, TNF-α, HA, PCⅢ and C-Ⅳ in patients with chronic hepatitis B were higher than those in controls, and increased with the severity of liver damage, and positively correlated with the degree of liver damage. And liver fibrosis has a significant correlation (P <0 05 or P <0 01). There was a linear correlation between serum TGF-β1 level and serum HA, PCⅢ and C-Ⅳ levels, while IFN-γ was negatively correlated with serum HA, PCⅢ and C-Ⅳ levels. Conclusion Serum levels of TGF-β1, TNF-α and IFN-γ in patients with chronic hepatitis B are related to liver fibrosis, and their serum levels can be used as a serological marker for diagnosing liver fibrosis