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目的:探讨竹节参皂苷Ⅳa对高脂饮食小鼠小肠干细胞及其微环境Wnt/β-catenin信号通路的影响。方法:C57BL小鼠雌雄各半随机分成4组,每组10只,即正常对照组、模型组及竹节参皂苷Ⅳa低、高剂量组。HE染色观察小肠形态学变化,免疫组化检测各组小肠干细胞数量、PCNA表达及其微环境Wnt/β-catenin信号通路的变化。结果:与正常对照组比较,模型组小肠绒毛长度、V/C值、PCNA表达量及杯状细胞数量、Bmi1阳性表达的小肠干细胞数量显著升高,小肠干细胞微环境Wnt/β-catenin信号显著上调(P<0.05或P<0.01)。与模型组比较,竹节参皂苷Ⅳa组绒毛长度、V/C值、PCNA表达量、杯状细胞数量、Bmi1阳性表达的小肠干细胞数量显著降低,小肠干细胞微环境Wnt/β-catenin信号显著下调(P<0.05或P<0.01)。结论:竹节参皂苷Ⅳa可通过Wnt/β-catenin信号通路调控小肠干细胞微环境,改善其过度增殖分化状态和抑制小肠干细胞增多,调节小肠干细胞功能。
OBJECTIVE: To investigate the effects of sTsP Ⅳ a on Wnt / β-catenin signaling pathway in small intestine stem cells and its microenvironment in high-fat diet mice. Methods: The male and female C57BL mice were randomly divided into 4 groups of 10 mice in each group, namely, the normal control group, the model group and the low and high dose group. The morphological changes of small intestine were observed by HE staining. The numbers of small intestine stem cells, expression of PCNA and Wnt / β-catenin signaling pathways in each group were detected by immunohistochemistry. Results: Compared with normal control group, the number of villus length, V / C, PCNA expression, goblet cells and Bmi1 expression of small intestine in model group were significantly increased. Wnt / β-catenin signal of small intestinal stem cell microenvironment was significantly (P <0.05 or P <0.01). Compared with model group, the length of villus, V / C value, PCNA expression, goblet cell number, the number of Bmi1 positive expression of small intestine stem cells were significantly decreased, and the Wnt / β-catenin signal of small intestinal stem cell microenvironment was significantly downregulated (P <0.05 or P <0.01). CONCLUSION: Stemona saponin Ⅳa can regulate the microenvironment of small intestine stem cells through Wnt / β-catenin signaling pathway, improve its over-proliferation and differentiation status and inhibit the growth of small intestine stem cells and regulate the function of small intestine stem cells.