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目的观察结核性胸膜炎患者外周血单个核细胞(PBMCs)中CD4 CD25调节性T细胞(CD4 CD25 Treg)水平及Foxp3 mRNA表达的变化,探讨CD4 CD25 Treg在结核性胸膜炎发病中的作用。方法采用流式细胞仪检测58例结核性胸膜炎患者和51例健康志愿者(正常对照组)PB-MCs中CD4 CD25 Treg的比例;RT-PCR检测PBMCs中Foxp3 mRNA的表达。结果结核性胸膜炎患者PBMCs中CD4 CD25 Treg的比例明显高于正常对照组(P<0.05);Foxp3 mRNA的表达明显高于正常对照组(P<0.05)。结论结核性胸膜炎患者外周血中具有免疫抑制活性CD4 CD25Treg的数量增加,功能增强,可能是结核性胸膜炎发生发展的一个因素。
Objective To observe the changes of CD4 CD25 Treg and Foxp3 mRNA expression in peripheral blood mononuclear cells (PBMCs) of patients with tuberculous pleurisy and to explore the role of CD4 CD25 Treg in the pathogenesis of tuberculous pleurisy. Methods Flow cytometry was used to detect the proportion of CD4 CD25 Tregs in PB-MCs from 58 patients with tuberculous pleurisy and 51 healthy volunteers (normal control group). The expression of Foxp3 mRNA in PBMCs was detected by RT-PCR. Results The percentage of CD4 CD25 Tregs in PBMCs was significantly higher in patients with tuberculous pleurisy than in normal controls (P <0.05). The expression of Foxp3 mRNA in PBMCs was significantly higher than that in controls (P <0.05). Conclusion The number of immunosuppressive CD4 CD25 Tregs in peripheral blood of patients with tuberculous pleurisy increased and the function of CD4 CD25 Tregs was enhanced, which may be one of the factors of the development of tuberculous pleurisy.