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本文用ANS荧光探剂,自旋标记探剂5NS的实验结果表明,L·(H~+-A7P酶)_(+Mg)~(2+)(Mg~(2+)存在下嵌有猪心线粒体H~+-ATP酶的脂酶体)的流动性小于L·(H~+-ATP酶)_(-Mg)~(2+)(无Mg~(2+)条件下组装的脂酶体)。DPH荧光偏振的测定也得到相似的结果。但自旋标记物12NS,16NS的实验结果反映两种脂酶体脂质分子的流动性并没有明显差异。这表明,Mg~(2+)能影响ATP酶重建体系中靠近表层部份脂质分子的流动性,而对较深层的流动性没有明显影响。可以设想,在Mg~(2+)存在下,重建猪心线粒体H~+-ATP酶活性的提高很可能是通过它对脂质分子靠近表层部份的物理状态的改变,继而影响疏水蛋白与F_1的构象,从而使H~+-ATP酶表现较高的活性。
In this paper, ANS fluorescence probe, spin-labeled probe 5NS experimental results show that, L · (H ~ + -A7Pase) _ (+ Mg) ~ (2 +) (Mg ~ (H ~ + -ATPase) _ (- Mg) ~ (2 +) (without Mg ~ (2+)) lipids of mitochondrial H ~ + -ATPase Body). DPH fluorescence polarization measurements also yielded similar results. However, the experimental results of spin labels 12NS and 16NS showed no significant difference in the fluidity of the two liposomes. This indicates that Mg 2+ can affect the fluidity of lipid molecules close to the surface layer in the ATPase reconstitution system and has no obvious effect on the deeper fluidity. It is conceivable that in the presence of Mg 2+, the enhancement of mitochondrial H ~ + -ATPase activity in porcine heart may be due to the change in its physical state near the surface of lipid molecules, which in turn affects the interaction between hydrophobin and F 1 conformation, so that H ~ + -ATPase showed higher activity.