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用荧光素双标记技术观察了大鼠大脑皮层、杏仁中央核和黑质网状带向下位脑干面口部运动核及其周围区域的分支投射。将ＦａｓｔＢｌｕｅ（ＦＢ）和ＤｉａｍｉｄｉｎｏＹｅｌｌｏｗ（ＤＹ）分别注入两侧脑干面口部运动核（三叉神经运动核、面神经核和舌下神经核）、左侧两个不同脑干面口部运动核、左侧和右侧两个不同胞干面口部运动核及其周围区域后，大脑皮层的ＦＢ／ＤＹ双标神经元占ＦＢ单标、ＤＹ单标和ＦＢ／ＤＹ双标神经元总数的１４．８％～３４．１％，杏仁中央核和黑质网状带的ＦＢ／ＤＹ双标神经元分别占标记神经元总数的４８．１％～７０．３％和２５．４～３５．８％。三叉神经运动核、面神经核和舌下神经核是主理咀嚼、吞咽、吸吮、表情和发声等复杂活动的运动神经元所在地，上述区域发出的轴突对这些运动核及其周围区域的分支支配，对面口部的横纹肌活动的精细调控和协调，可能有重要作用和意义。
Fluorescein double labeling technique was used to observe the branching projection of the motor cortex, almond central nucleus and substantia nigra mesh zone to the lower level brainstem facial nucleus and the surrounding area. FastBlue (FB) and DiamidinoYellow (DY) were injected into the motor nuclei (the trigeminal motor nucleus, the facial nucleus and the sublingual nucleus) on both sides of the brainstem, The FB / DY double-labeled neurons in the cerebral cortex accounted for 14% of the total number of FB single-labeled, DY single-labeled and FB / DY double-labeled neurons after lateral and right facial nuclei and their surrounding areas. 8% -34.1%. The FB / DY double-labeled neurons in central amygdala and substantia nigra reticulae accounted for 48.1% -70.3% and 25.4-35.8% of the total number of labeled neurons, respectively . The trigeminal motor nucleus, the facial nucleus and the sublingual nucleus are the loci of motor neurons that are responsible for complex activities such as chewing, swallowing, sucking, expression and vocalization. The axons in these regions control the branches of these motor nuclei and their surrounding areas , The fine control and coordination of rhabdomyra activity across the mouth can have an important role and significance.