论文部分内容阅读
背景:血管内皮功能紊乱在血管性头痛的发生发展过程中起重要作用,一氧化氮、内皮素可能参与偏头痛的病理生理过程,改善偏头痛患者一氧化氮、内皮素水平的异常有助于偏头痛的治疗。目的:观察颅痛安颗粒对大鼠血管内皮细胞功能的影响,探讨其治疗头痛的机制。设计:随机对照的实验研究。单位:泸州医学院药学院药理教研室,中山大学医学院第一附属医院烧伤科,潍坊市人民医院心内科。材料:实验在泸州医学院药学院药理实验室完成,选择SD大鼠50只,随机分为正常对照组,模型组,颅痛安颗粒大剂量(4g/kg)组,颅痛安颗粒中剂量(2g/kg)组,颅痛安颗粒小剂量(1g/kg)组,每组10只。干预:复制血管内皮细胞功能紊乱动物模型后治疗5d,取血测组织型酶原激活物(t-PA)活性,纤溶酶原激活物抑制剂-1(PAI-1)活性、一氧化氮及血管内皮素含量,求活性t-PA百分率。主要观察指标:t-PA活性、PAI-1活性、一氧化氮和内皮素含量。结果:模型组较正常对照组t-PA活性[(2.0123±0.3422)IU/mL比(4.2199±0.4924)IU/mL]、活性型t-PA百分率[(14.5937±1.5196)%比(35.1608±3.1152)%]、一氧化氮含量[(2.2873±0.3081)mol/L比(2.7371±0.4182)mol/L]均显著降低(P<0.05),PAI-1活性[(14.8895±2.0971)AU/mL比(7.8239±0.5211)AU/mL]、内皮素含量[(3.1771±
BACKGROUND: Endothelial dysfunction plays an important role in the development of vascular headache. Nitric oxide and endothelin may be involved in the pathophysiological process of migraine. It is helpful to improve the abnormal levels of nitric oxide and endothelin in migraine patients. Treatment of migraine. OBJECTIVE: To observe the effect of Shoutongan Granule on the function of rat vascular endothelial cells and to explore its mechanism of headache treatment. Design: Randomized controlled experimental study. Unit: Department of Pharmacology, School of Pharmacy, Luzhou Medical College, Department of Burns, First Affiliated Hospital, School of Medicine, Sun Yat-sen University, Department of Cardiology, People’s Hospital of Weifang City. MATERIALS: The experiment was performed in the pharmacology laboratory of the Pharmaceutical College of Luzhou Medical College. Fifty SD rats were randomly divided into normal control group, model group, high dose (4g/kg) group of granules of granules, and granules in the middle group In the (2g/kg) group, a small dose (1g/kg) group of Shouganian Granules was used, and 10 rats in each group. Intervention: After 5 days of treatment of animal models of vascular endothelial dysfunction, blood was taken to measure tissue-type plasminogen activator (t-PA) activity, plasminogen activator inhibitor-1 (PAI-1) activity, and nitric oxide And vascular endothelin content, find the percentage of active t-PA. MAIN OUTCOME MEASURES: t-PA activity, PAI-1 activity, nitric oxide and endothelin content. Results: Compared with the normal control group, t-PA activity in the model group [(2.0123±0.3422) IU/mL ratio (4.2199±0.4924) IU/mL] and the percentage of active t-PA [(14.5937±1.5196)%] (35.1608±3.1152) )%], Nitric oxide content [(2.2873±0.3081) mol/L ratio (2.7371±0.4182) mol/L] were significantly lower (P<0.05), PAI-1 activity [(14.8895±2.0971) AU/mL ratio (7.8239±0.5211) AU/mL], endothelin content [(3.1771±)