论文部分内容阅读
[目的]研究羟基磷灰石（HAP）纳米粒子对肝癌细胞和肝细胞的选择性作用。[方法]以细胞系Bel-7402人肝癌细胞和大鼠肝细胞为对象,倒置显微镜下观察细胞的生长状态,应用活细胞计数法绘制生长曲线。[结果]经HAP纳米粒子（1.4×10-4mol/L）作用的肝癌细胞与其对照组相比,细胞数量逐渐减少,胞质空泡样变,二者存在显著性差异（P<0.01）。而加入不同浓度HAP纳米粒子的肝细胞组,存活细胞数量明显增多,与对照组之间无明显差异。[结论]HAP纳米粒子有明显的体外抗肝癌作用,而对正常肝细胞影响轻微。
[Objective] To study the selective effect of hydroxyapatite (HAP) nanoparticles on hepatoma cells and hepatocytes. [Method] Bel-7402 human hepatocarcinoma cells and rat hepatocytes were used as target. The growth of cells was observed under inverted microscope. The growth curve was drawn by living cell counting. [Results] Compared with the control group, the number of HCC nanoparticles (1.4 × 10-4 mol / L) decreased gradually and the number of cytoplasm vacuolization changed significantly (P <0.01). However, the number of viable cells in HAP nanoparticles with different concentrations of HAP nanoparticles increased significantly compared with the control group. [Conclusion] HAP nanoparticles have obvious anti-HCC effect in vitro, but have little effect on normal liver cells.