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目的 :了解我国人群中是否存在SEN病毒 (SENV)感染 ,并分析SENV国内分离株的部分基因序列。方法 :在SENV的保守区设计引物 ,建立巢式PCR方法检测血清中SENVDNA ,并对PCR产物进行克隆、测序。结果 :7例非甲～非庚型肝炎 ,且输血传播病毒 (TTV)阴性患者中 ,2例SENV阳性。其中一株的序列与意大利SENV D株 (AX0 2 5730 )相对应位置核苷酸序列同源性为90 %。结论 :本研究证实了我国存在SENV感染 ,建立了检测SENV的PCR方法 ,并对SENV部分基因进行了克隆及测序 ,对进一步开展SENV诊断和流行病学调查具有重要意义
Objective: To know if there is SENV infection in our population and to analyze the partial gene sequences of SENV isolates in China. Methods: Primers were designed in conserved regions of SENV, and nested PCR was used to detect SENVDNA in serum. The PCR products were cloned and sequenced. Results: Of the 7 non-A to non-G hepatitis patients, 2 were SENV positive in patients with negative TTV transfusion. One of them had 90% nucleotide sequence homology with that of SENV D strain (AX0 2 5730) in Italy. Conclusion: This study confirms the presence of SENV infection in our country and establishes a PCR method for detecting SENV. Cloning and sequencing of some SENV genes are of great significance for the further diagnosis and epidemiological investigation of SENV