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目的 研究沙土鼠短暂的脑缺血后海马CA1区Bcl - 2蛋白的表达 ,探讨Bcl- 2蛋白表达与脑缺血耐受之间的关系。方法 采用夹闭沙土鼠双侧颈总动脉造成脑缺血模型。 6 3只健康沙土鼠随机分成四组。非缺血对照组 (A组 ,n =5 ) ;预处理对照组 (B组 ,n =6 ) ,单次脑缺血 2min ;缺血预处理组 (PC组 ,n =2 6 )与缺血对照组 (IR组 ,n =2 6 ) ,有或无预处理缺血 2min ,间隔 3d后缺血5min ,分别再灌 4h (PC1组 ,n =5 ;IR1组 ,n =5 )、2 4h (PC2组 ,n =7;IR2组 ,n =7)、72h(PC3组 ,n =7;IR3组 ,n =7)、7d(PC4组 ,n =7;IR4组 ,n =7) ,实验终末 ,断头取脑 ,作海马石蜡切片 ,免疫组化染色评定Bcl 2蛋白免疫反应强度。结果 B组的Bcl 2蛋白免疫反应强度比A组明显提高 (P <0 0 1) ;PC1组的Bcl 2蛋白免疫反应强度强于IR1组 (P <0 0 5 ) ,PC3组强于IR3组 (P <0 0 1) ;PC4组强于IR4组 (P <0 .0 5 ) ;但PC2组的Bcl 2蛋白免疫反应强度与IR2组比较无显著差别 (P >0 0 5 )。结论 缺血预处理后CA1区Bcl 2蛋白表达增强与缺血耐受的获得有关。
Objective To study the expression of Bcl - 2 protein in hippocampal CA1 region after transient cerebral ischemia in gerbils and to explore the relationship between Bcl - 2 protein expression and cerebral ischemic tolerance. Methods The bilateral common carotid arteries were used to occlude the cerebral ischemia model. Six healthy gerbils were randomly divided into four groups. The rats in the non-ischemic control group (group A, n = 5), preconditioning control group (group B, n = 6), single cerebral ischemia for 2 minutes, ischemic preconditioning group (PC group, n = 26) The rats in control group (IR group, n = 26) were given ischemia with or without preconditioning for 2 minutes and ischemia for 5 minutes after 3 days and reperfusion for 4 hours (PC1 group, n = 5; IR1 group, n = 5) (PC2 group, n = 7; IR2 group, n = 7), 72h (PC3 group, n = 7; IR3 group, n = 7) At the end of the experiment, the brain was decapitated and paraffin sections of the hippocampus were obtained. Immunohistochemical staining was used to evaluate the intensity of Bcl 2 protein immunoreactivity. Results The Bcl 2 protein immunoreactivity in group B was significantly higher than that in group A (P <0.01). The Bcl 2 protein immunoreactivity in PC1 group was stronger than that in IR1 group (P <0 05) (P <0.01). The PC4 group was stronger than the IR4 group (P <0.05). However, there was no significant difference in the Bcl 2 immunoreactivity between PC2 group and IR2 group (P> 0.05). Conclusion The increased expression of Bcl-2 in CA1 area after ischemic preconditioning is associated with the development of ischemic tolerance.