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目的 探讨广西 1997～ 2 0 0 1年霍乱流行菌株的分子生物学特征以及他们之间的内在联系及其流行特点。方法 采用打点杂交方法检测霍乱弧菌毒力相关基因ctxAB、zot,运用 16srRNA探针进行核糖体基因分型。结果 O13 9群霍乱弧菌ctxAB、zot基因携带率为 10 0 % ,埃尔托霍乱弧菌ctxAB基因携带率为 97 6 7% ,zot基因携带率为 95 35 % ;用 16srRNA探针分型可分为 6个核糖体基因型 (RT1～RT6) ,以RT1和RT4为主要型别。结论 普遍携带ctxAB、zot基因 ,具有较强的致病性。 1997～ 2 0 0 0年与 2 0 0 1年分离的菌株在核糖体杂交图谱上有明显的差异 ,同一核糖体型可以来源于不同的血清型和噬菌体 -生物型的菌株。分析认为 2 0 0 1年的稻叶型菌株不是 2 0 0 0年稻叶型菌株的延续 ,而是一个新的克隆群。
Objective To investigate the molecular biological characteristics of cholera epidemic strains in Guangxi from 1997 to 2001 and their internal relations and their epidemiological characteristics. Methods Dot-blot hybridization was used to detect the virulence-associated genes of V. cholerae ctxAB and zot, and ribosomal genotyping was performed using 16srRNA probe. Results The prevalence of ctxAB and zot genes in Vibrio cholerae O13 9 strain was 100%. The carriage rate of ctxAB gene in V. cholerae was 97 6 7% and the carriage rate of zot gene was 95 35%. The 16s rRNA probe typing Divided into 6 ribosomal genotypes (RT1 ~ RT6), with RT1 and RT4 as the main type. Conclusion Common carrying ctxAB, zot gene, has a strong pathogenicity. The strains isolated from 1997 to 2000 and 2001 have obvious differences in the ribosomal hybridization map. The same ribosomal type can be derived from different serotypes and bacteriophage-biotype strains. The analysis shows that in 2001, the rice leaf-type strain is not a continuation of the rice leaf-type strain in 2000, but a new clonal group.