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目的研究番泻叶不同粉体的化学成分与泻下作用。方法以燥结缺水便秘小鼠为模型,运用色素流动法,以黑便为指示,通过观测小鼠首次排黑便的时间、稀便点数等指标来比较说明番泻叶不同粉体的泻下作用强弱。利用HPLC法测定番泻叶不同粉体致泻主要活性成分番泻苷A、番泻苷B,比较各粉体中番泻苷A、B的量高低,反映药理活性强弱。结果通过小鼠动物实验观测到,番泻叶超微饮片组首次排黑便的时间明显提前,比饮片组提前了76min,比超微粉组提前了18min;稀便点数增多,比药材饮片组多3.7个,比超微粉组多2.84个。HPLC法测定番泻苷A、B的量显示,超微饮片中番泻苷A和B的质量分数为0.43%,是药材饮片中的1.6倍。结论番泻叶3种粉体的水煎液都能明显增加小鼠腹泻的发生率,尤其以番泻叶超微饮片的通便泻下作用最显著,番泻苷A、B的量最高。
Objective To study the chemical constituents and diarrhea effects of different powders in senna. Methods Dry mice with constipation and dehydration constipation were used as a model. The color flow method was used to indicate the presence of black stools. By observing the time of first stool excretion and the number of loose stools in mice, Under the effect of strength. The main active components of diarrhea Senna glycoside A and sennoside B were determined by HPLC. The contents of sennoside A and B in each powder were compared to determine the pharmacological activity. Results The results of animal experiments in mice showed that the time for the first batch of melena in senna ultramicro decoction group was significantly earlier than that in the decoction group for 76 minutes and 18 minutes earlier than that in the superfine powder group, 3.7, more than 2.84 superfine powder group. The determination of the amount of sennoside A and B by HPLC showed that the mass fraction of sennoside A and B in the ultramicro pieces was 0.43%, which was 1.6 times of that in the medicinal herbs. Conclusions The decoction of senna powder can significantly increase the incidence of diarrhea in mice. Especially senna purpurea laxative effect is the most significant, Sennoside A, B the highest.