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目的：研究粘附肽ＲＧＤ（精氨酸－甘氨酸－天冬氨酸）对小鼠成骨细胞在材料表面附着和铺展的影响，为使用粘附肽对种植材料进行表面修饰提供体外实验依据。方法：采用自组装单层结构法将含有ＲＧＤ的粘附肽片段ＲＧＤＣ以共价键固定在材料表面。小鼠成骨细胞体外培养４ｈ，细胞计数分析其对材料的附着。ＦＩＴＣ标记免疫荧光染色观察培养４ｈ后细胞内肌动蛋白的分布。结果：ＲＧＤ可以使细胞对材料表面的附着密度显著提高。体外培养４ｈ，细胞在ＲＧＤ材料表面具有良好的铺展形态。结论：表面固定 ＲＧＤ可以促进细胞对材料表面的附着和铺展。
OBJECTIVE: To study the effect of RGD (arginine-glycine-aspartic acid) on the adhesion and spreading of mouse osteoblasts on the surface of the material, and to provide an experimental basis for the surface modification of the implant material using the adhesive peptide. Methods: The self-assembled monolayer method was used to attach the RGD-containing RGD to the surface of the material by covalent bonding. Mouse osteoblast cultured in vitro 4h, cell count analysis of its attachment to the material. FITC labeling immunofluorescence staining observed after 4h cultured cells actin distribution. Results: RGD could significantly increase the cell attachment density on the surface of the material. In vitro cultured 4h, the cells in the RGD material surface has a good spreading morphology. Conclusion: Surface-immobilized RGD can promote cell attachment and spreading on the surface of the material.