论文部分内容阅读
以延边地区苹果梨为试材,采用同源克隆及实时荧光定量PCR的试验方法,研究PyANS基因在苹果梨解袋前后果实着色过程中的表达特性及与花青苷积累的关系。结果表明:PyANS的全长cDNA为1 075bp,其开放阅读框(ORF)编码358个氨基酸,分子量约为40kDa,理论等电点pI为5.38。同源性分析表明与同属的砂梨相似性高达99%,实时荧光定量PCR分析表明PyANS基因受到光诱导表达量迅速上升,去袋1d后表达量迅速增加,第10天时达到最大值,随后迅速下降,最终稳定在同一表达水平,与果实着色和花色苷含量测定的结果相对应,表明PyANS基因对果实花色素苷积累有一定的促进作用。
Using the apple-pear cultivars in Yanbian area as experimental materials, the expression characteristics of PyANS gene during fruit coloring process before and after unpacking and the relationship with anthocyanin accumulation were studied by using homologous cloning and real-time fluorescence quantitative PCR. The results showed that the full-length cDNA of PyANS was 1 075 bp with an open reading frame (ORF) encoding 358 amino acids with a molecular weight of about 40 kDa and a theoretical pI of 5.38. Homology analysis showed that 99% similarity with the same sand pear, real-time PCR analysis showed that the light-induced expression of PyANS gene increased rapidly after bag removal 1d the expression increased rapidly, reaching the maximum on the 10th day, then Decreased rapidly and finally stabilized at the same level of expression, corresponding to the results of fruit coloring and anthocyanin content determination, indicating that PyANS gene can promote fruit anthocyanin accumulation to a certain extent.