论文部分内容阅读
目的:建立快速的序列特异性聚合酶链反应(SSP-PCR)方法,检测人多药耐药基因C3435T、G2677T两个单核苷酸多态性。方法:优化SSP-PCR反应条件,将其结果与DNA测序进行比对,确定其准确性。采集158例汉族正常人的外周血,检测MDR1C3435T和G2677T基因的变异情况。结果:优化后SSP-PCR结果与测序结果相符合。158例个体C3435T基因分布,C纯合子占37.34%,T纯合子占11.39%,C/T杂合子占51.27%。G2677T基因分布,G、T、A等位基因出现频率分别为44.30%、39.24%、16.46%。汉族人中此两位点多态性分布与高加索人存在显著差异(P<0.05)。结论:本研究成功建立了SSP-PCRMDR1C3435T、G2677T单核苷酸多态性的快速分型技术。
Objective: To establish a rapid sequence-specific polymerase chain reaction (SSP-PCR) method to detect two single nucleotide polymorphisms of human multidrug resistance gene C3435T and G2677T. Methods: The conditions of SSP-PCR reaction were optimized, and the results were compared with DNA sequencing to determine its accuracy. Peripheral blood samples from 158 Han normal subjects were collected to detect the variation of MDR1C3435T and G2677T genes. Results: The optimized SSP-PCR results were in accordance with the sequencing results. 158 cases of C3435T gene distribution, C homozygotes accounted for 37.34%, T homozygotes accounted for 11.39%, C / T heterozygotes accounted for 51.27%. G2677T gene distribution, G, T, A allele frequencies were 44.30%, 39.24%, 16.46%. There was a significant difference between the two loci in Han nationality and Caucasians (P <0.05). Conclusion: This study successfully established SSP-PCRMDR1C3435T, G2677T single nucleotide polymorphism rapid typing technology.