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目的 研究多药耐药 (MDR- 1)基因在急性白血病 (AL)治疗中的表达。方法 用逆转录 -多聚酶链反应 (RT- PCR)技术检测 4 7例 AL的 87份骨髓血标本。结果 1MDR- 1基因阳性表达率在 4 0例初诊和 2 2例完全缓解 (CR)的病例中分别为 2 2 .5%和 13.6 % ,显著低于未缓解及复发者 (11例 )和死亡前期者 (14例 )的中数值 (72 .7%和 78.6 % ,P<0 .0 1)。 2 CR率在其表达阴性的初诊者 (31例 )中高于其表达阳性者 (9例 ) (6 7.7% ,2 2 .2 % ,P=0 .0 53)。结论 MDR- 1基因表达检测对 AL的预后判断和指导选择治疗方案十分有意义
Objective To investigate the expression of multidrug resistance (MDR-1) gene in acute leukemia (AL). Methods Reverse transcription polymerase chain reaction (RT-PCR) was used to detect 87 bone marrow samples from 47 AL patients. Results The positive rate of 1MDR-1 gene was 22.5% and 13.6% in 40 new cases and 22 cases of complete remission (CR) respectively, which were significantly lower than those in unrelated and recurrent cases (11 cases) and death Median values of pre-term (14 patients) (72.7% vs 78.6%, P <0.01). 2 CR rate was higher in newly diagnosed patients (31 cases) than in those with positive expression (9 cases) (6 7.7%, 22.2%, P = 0.053). Conclusion The detection of MDR-1 gene expression is of great significance for the prognosis of AL and for guiding the choice of treatment