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目的探讨染砷大鼠脑组织中砷代谢产物与砷代谢相关酶的性别差异。方法将70只健康7周龄清洁级Wistar大鼠随机分为7组,分别为对照(去离子水)组及20、6.7、2.2 mg/kg亚砷酸钠和砷酸钠染毒组,每组10只,雌雄各半。采用自由饮水方式进行染毒,连续染毒3个月。测定大鼠脑组织中iAs3+、iAs5+、一甲基胂酸(MMA)、二甲基胂酸(DMA)含量及砷代谢相关酶甲基转移酶(As3mt)活力和谷胱甘肽(GSH)含量。结果各染毒组均未检出iAs5+。与对照组比较,除雌性20 mg/kg亚砷酸钠和砷酸钠染毒组大鼠脑组织中iAs3+含量低于对照组外,雌、雄其他各染毒组iAs3+含量均高于对照组(P<0.05)。与雌性大鼠比较,雄性大鼠20 mg/kg亚砷酸钠和砷酸钠染毒组脑组织中iAs3+含量较高,其他各染毒组雄性iAs3+含量均较低(P<0.05)。与对照组比较,除雌性2.2 mg/kg砷酸钠染毒组及雄性2.2 mg/kg亚砷酸钠、6.7 mg/kg砷酸钠染毒组大鼠脑组织中DMA含量未检出外,其他雌雄各剂量组有机砷含量均高于对照组(P<0.05)。与雌性大鼠比较,雄性2.2、20 mg/kg亚砷酸钠染毒组和6.7、20 mg/kg砷酸钠染毒组大鼠脑组织中DMA含量较低,雄性亚砷酸钠2.2 mg/kg及砷酸钠6.7、20 mg/kg染毒组大鼠脑组织中MMA含量较高(P<0.05)。与对照组比较,雌性2.2 mg/kg亚砷酸钠、各剂量砷酸钠染毒组以及雄性各剂量染毒组大鼠脑组织中As3mt活力均较高,雄性2.2 mg/kg、6.7 mg/kg亚砷酸钠染毒组和2.2 mg/kg砷酸钠染毒组以及雌性各剂量染毒组大鼠脑组织中GSH含量均较低(P<0.05)。与雌性大鼠比较,雄性仅2.2 mg/kg亚砷酸钠染毒组大鼠脑组织中As3mt活力和雄性6.7 mg/kg亚砷酸钠染毒组大鼠脑组织中GSH含量较低(P<0.05)。结论大鼠脑砷代谢过程中代谢产物及代谢酶雌雄间有明显差异,可能与大鼠脑砷代谢基础及脑砷甲基化能力雌雄间差异有关。
Objective To investigate the gender differences in arsenic metabolism related enzymes and arsenic metabolism in arsenic-exposed rat brain. Methods Seventy healthy Wistar rats, aged 7 weeks, were randomly divided into 7 groups: control (deionized water) and 20, 6.7, 2.2 mg / kg sodium arsenite and sodium arsenate Group of 10, male and female. Using free drinking water for exposure, continuous exposure for 3 months. The contents of iAs3 +, iAs5 +, MMA, DMA and As3mt activities and glutathione (GSH) contents in rat brain were measured. . Results No iAs5 + was detected in all the infected groups. Compared with the control group, the content of iAs3 + in brain tissues of female rats exposed to 20 mg / kg sodium arsenite and sodium arsenate was lower than that of the control group (P <0.05). Compared with female rats, the content of iAs3 + in brain tissue of 20 mg / kg sodium arsenite and sodium arsenate rats was higher in male rats, and the content of male iAs3 + in each group was lower (P <0.05). Compared with the control group, in addition to the female 2.2 mg / kg sodium arsenate exposure group and male 2.2 mg / kg sodium arsenite, 6.7 mg / kg sodium arsenate exposure in rats brain tissue DMA content was not detected, The concentrations of organic arsenic in other male and female groups were higher than those in the control group (P <0.05). Compared with female rats, the content of DMA in the brain of rats exposed to 2.2,20 mg / kg sodium arsenite and 6.7,20 mg / kg sodium arsenate was lower than that of the female rats, and the concentration of maleate sodium arsenite was 2.2 mg / kg and sodium arsenate 6.7, 20 mg / kg in the rat brain tissue MMA content was higher (P <0.05). Compared with the control group, the As3mt activity of 2.2 mg / kg female sodium arsenite, sodium arsenite exposure groups and male rats in each dose group were higher than the control group, and the male 2.2 mg / kg, 6.7 mg / kg sodium arsenite exposure group and 2.2 mg / kg sodium arsenate exposure group and the female rats in each dose exposure had a lower GSH content (P <0.05). Compared with female rats, GSH levels in brain tissue of male rats exposed to sodium arsenite at a concentration of 2.2 mg / kg were lower than those exposed to sodium arsenite at a concentration of 6.7 mg / kg <0.05). Conclusions There is a significant difference between the metabolic products and the metabolic enzymes in the cerebral arsenic metabolism in rats, which may be related to the basis of cerebral arsenic metabolism in rats and the difference in the capacity of brain arsenic methylation.