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在扫描电镜观察大多数制样采用临界点干燥法。这里向大家介绍的是用HMDS法,这一新的方法的全过程,包括组织先在3.1％戊二醛溶液中固定,再用1％锇酸后固定,并进行系列乙醇脱水,HMDS干燥,再离子测射,最后通过扫描电镜观察。我们经过对小鼠的脏器,兔的主动脉,培养细胞以及立克次氏体等样品的制备,并通过扫描电镜的观察,发现不仅在样品的表面形态,而且在微细结构方面均与临界点干燥法无明显差别。常规干燥方法,采用临界点干燥法,它需要临界点干燥仪,还需要高纯度二氧化碳。在夏季特别是室温超过30℃时,液态CO_2难于装满干燥仪的样品室。因此,在样品室加热时,CO_2液面要穿过样品,
In the SEM observation of most of the sample preparation using critical point drying method. HMDS method introduced here, the whole process of this new method, including the organization fixed in 3.1% glutaraldehyde solution, and then fixed with 1% osmium tetroxide, and a series of ethanol dehydration, HMDS drying, Then ionizing radiation, and finally observed by scanning electron microscopy. Through the preparation of the visceral organs of mice, the aorta of rabbits, cultured cells and rickettsiae, we observed by scanning electron microscopy that we found that both the surface morphology of the sample and the microstructure are related to the critical Point drying method no significant difference. Conventional drying methods, using critical point drying method, which requires a critical point dryer, but also requires high purity carbon dioxide. In summer, especially when the room temperature exceeds 30 ℃, the liquid CO_2 is difficult to fill the sample chamber of the dryer. Therefore, when the sample chamber is heated, the CO 2 level passes through the sample,