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【目的】建立黄芪散复方中葛根与桑白皮的质量控制方法。【方法】以桑根酮D、葛根素、1-脱氧野尻霉素(DNJ)为对照品,采用高效液相色谱(HPLC)法测定黄芪散质量。【结果】HPLC法检测桑根酮D、葛根素、1-脱氧野尻霉素(DNJ),各有效成分完全分离,峰面积与其质量浓度呈良好的线性关系,加样回收率分别为99.2%、98.6%、99.6%;相对标准偏差(sR)分别为1.97%、1.58%、1.81%。【结论】该含量测定方法准确、简便、重复性好,是一种较理想的控制黄芪散复方质量的方法。
【Objective】 To establish a method for quality control of Radix Puerariae and Morus alba in Radix Astragali. 【Method】 Morus alba root D, Puerarin and 1-deoxynojirimycin (DNJ) were used as reference substance, and the quality of Radix Astragali was measured by high performance liquid chromatography (HPLC). 【Result】 Sanggenone D, puerarin and 1-deoxynojirimycin (DNJ) were detected by HPLC. The active components were completely separated and the peak area was linear with the mass concentration. The recoveries were 99.2% 98.6% and 99.6%, respectively. The relative standard deviations (RSDs) were 1.97%, 1.58% and 1.81% respectively. 【Conclusion】 This method is accurate, simple and reproducible. It is an ideal method to control the quality of Radix Astragali.