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将甘油经静脉注入实验动物,研究其对内淋巴囊(ES)上皮细胞活性和ES容量的长期影响。其方法是取38只重约20～25g成年健康NMRI鼠,其中28只经尾静脉注入甘油,1分钟处死1只动物,以后分别在不同的时间(15,30,60分钟,2,4,6,8小时和2,7天)各处死3只动物;另10只作为对照组,静脉注入生理盐水,并在相同间隔时间各处死1只,然后按照Rask-Anderson等的方法解剖和处理颞骨。将ES内皱褶部分作成薄切片和超薄切片,采用显微照像机和透射电镜观察分析,包括:①用药后不同时间ES闭塞或扩张的
Glycerol was injected intravenously into experimental animals to investigate its long-term effects on the activity of the endolymphatic sac (ES) epithelial cells and ES volume. The method comprises the following steps: taking 38 healthy adult healthy NMRI mice weighing about 20-25g, of which 28 are injected via the tail vein with glycerol and one animal is sacrificed at one minute, and then at different times (15, 30, 60 minutes, 2, 4, 6, 8 and 2, 7 days). Three animals were sacrificed at the same time. The other 10 rabbits were injected with normal saline intravenously and sacrificed one at the same interval. The temporal bone was dissected and treated according to the method of Rask-Anderson et al . The ES part of the folds made thin sections and ultra-thin sections, the use of microscopic and transmission electron microscopy analysis, including: ① after administration of different times of ES occlusion or expansion